20.500.12556/DKUM-89677 Priprava organogelov z ekstrakti kurkume z optimalnim profilom sproščanja Formulation of organogels with turmeric extracts with optimal dissolution profile Namen magistrskega dela je izolacija in določitev biološko aktivnih komponent (kurkuminoidov) v ekstraktih kurkume, formulacija ekstrahiranih učinkovin s ciljem čim večje vsebnosti kurkumina in preverjanje protimikrobnega potenciala dobljenih ekstraktov in formulacij. Izvedli smo dve različni ekstrakcijski metodi. Pri ultrazvočni ekstrakciji smo kot topilo uporabili etanol, medtem ko smo superkritično ekstrakcijo izvedli s superkritičnim ogljikovim dioksidom. Nastalim ekstraktom smo določili antioksidativno aktivnost z metodo DPPH· ter vsebnosti totalnih fenolov, proantocianidinov, karotenoidov in vitamina C. Ekstrakte smo nato formulirali v organogele in enkapsulate. Vsebnost kurkumina smo določili v ekstraktih in organogelih, njegovo topnost v superkritičnem ogljikovem dioksidu pa smo preverili z uporabo visokotlačne celice pri različnih tlakih. Na koncu je sledila še analiza protimikrobnega potenciala, s katero smo pripravljenim ekstraktom in formulacijam določili minimalno inhibitorno koncentracijo. Iz rezultatov je razvidno, da je najvišji ekstrakcijski izkoristek pri ultrazvočni ekstrakciji (5,77 %). Prav tako smo temu ekstraktu, v primerjavi s superkritičnim, spektrofotometrično določili višji % inhibicije (41,93 %) in višje vsebnosti totalnih fenolov (2,06 mg GA/g materiala), proantocianidinov (0,6 mg PAC/g materiala) ter vitamina C (0,20 mg vitamina C/g materiala). Tudi vsebnosti kurkumina so višje v ultrazvočnem ekstraktu. Ultrazvočni ekstrakt in njegove formulacije imajo nižjo minimalno inhibitorno koncentracijo kot superkritični ekstrakt in njegove formulacije. Najnižnjo minimalno inhibitorno koncentracijo (0,47 mg/mL) smo določili ultrazvočenmu ekstraktu pri uporabi bakterije Staphylococcus aureus in glive Candida albicans. S tem smo dodatno potrdili višje vsebnosti kurkumina v ultrazvočnem ekstraktu in tudi to, da kurkumin deluje protimikrobno. The aim of this master thesis is the isolation and determination of biologically active components (curcruminoids) of turmeric extracts, the formulation of the extracted active compounds with the aim to maximise the curcumin content and to verify the antimicrobial potential of the produced extracts and formulations. Two different extraction methods were perfomed. In ultrasound extraction, ethanol was used as a solvent, while supercritical extraction was performed using supercritical carbon dioxide. The resulting extracts were analysed for their antioxidant activity using the DPPH method and determined for total phenols, proanthocyanidins, carotenoids and vitamin C. The extracts were then formulated into organogels and encapsulates. The curcumin content was determined in the extracts and organogels, and its solubility in supercritical carbon dioxide was tested using a high pressure cell at different pressures. Finally, an analysis of the antimicrobial potential of the prepared extracts and formulations was performed to determine the minimum inhibitory concentration. The results show that the maximum extraction yield is achieved with ultrasound extraction (5,77 %). We also spectrophotometrically determined that this extract, compared to the supercritical extract, has higher % inhibition (41,93 %) and higher content of total phenolics (2,06 mg GA/g materiala), proanthocyanidins (0,6 mg PAC/g materiala) and vitamin C (0,20 mg vitamina C/g materiala). The curcumin content is also higher in the ultrasound extracts. The ultrasound extract and its formulations have a lower minimum inhibitory concentration than the supercritical extract and its formulations. The lowest minimum inhibitory concentration (0,47 mg/mL) was determined for the ultrasound extract using Staphylococcus aureus and Candida albicans. Thus, we have further confirmed that the curcumin content is higher in the ultrasound extracts and that curcumin has antimicrobial activity. kurkuma kurkumin ekstrakcija organogeli enkapsulacija antimikrobni potencial turmeric curcumin extraction organogels encapsulation antimicrobial potential true true false [N. Šiler] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-08-04 01:18:16 2024-09-10 08:28:48 2024-11-13 14:34:31 0000-00-00 00:00:00 2024 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.061.3:633.826(043.2) 214505731 MAG_Siler_Nastja_2024.pdf MAG_Siler_Nastja_2024.pdf 1 70570394F09297EAEAF923C37235962B 7977914c5b1aacf92cbd7d5e5f59ecb7b0e0d5dd8982160674a1d72a1ae9bceb a6a60574-6eb1-11ef-a573-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=184725 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0