20.500.12556/DKUM-89713 Covalent modification of chitosan surfaces with a sugar amino acid and lysine analogues This work explores the modifcation and characterization of chitosan thin flms as a model for functionalized polysaccharide interfaces. The solid–liquid interface of oligo- and polysaccharides is crucial for various biological processes such as cell adhesion and recognition. By covalent surface modifcation of the chitosan via amide formation with diferent small molecules containing carboxylic acids, e.g. specially designed glycoside hydrolase inhibitors, interactions with biomolecules and living cells could potentially be controlled in the future. As a frst step towards this aim, three fuorescent compounds were conjugated onto nanometric chitosan thin flms. The layers were analysed by fuorescence spectroscopy, X-ray photoelectron spectroscopy, time-of-fight secondary ion mass spectrometry, and atomic force microscopy, to proof the covalent attachment of the target molecules. By this analysis, a uniform and chemically stable covalent attachment of the target molecules on the chitosan thin flms could be demonstrated under various conditions. This publication serves as a proof-of-concept-study for further biofunctionalization, pattering, and interaction studies involving polysaccharide interfaces, glycosidase inhibitors, proteins, or living cells. carbohydrates fluorescence spectroscopy amino acid chitosan thin flm modifcation C-Glycosides kemija karbohidrati amino kisline spektroskopija true false true Springer Angleški jezik Slovenski jezik https://link.springer.com/journal/706 © The Author(s) 2024 Članek v reviji 2024-08-07 08:53:47 2024-08-07 08:53:49 2026-01-16 12:09:20 0000-00-00 00:00:00 2024 0 0 11 str. Published: 03 August 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2023-12-19 2023-06-12 2024-08-03 54 203801859 10.1007/s00706-024-03227-y 1434-4475 s00706-024-03227-y.pdf s00706-024-03227-y.pdf 1 A9E85EB198D07487654B27D48DE5A3D1 6ed1ea65f4ca61aeb7756fb410c93f598072bcf6fc6c5dbae2186d4d16ac951c 4257453a-548a-11ef-b534-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=182071 https://link.springer.com/article/10.1007/s00706-024-03227-y#Sec15 0 66e850b2-f2cb-11f0-adbf-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=202092 Fakulteta za kemijo in kemijsko tehnologijo Fakulteta za strojništvo 0 0 0