20.500.12556/DKUM-89746 Synthesis, purification, and cell-toxicity of a choline betainate In this work, choline chloride and betaine hydrochloride were condensed into a - to our knowledge - unreported choline betainate (N,N,N-trimethyl-2-oxo-2-(2-(trimethylammonio)ethoxy)ethanaminium chloride) using 1,1′-carbonyldiimidazole (CDI) activation of betaine hydrochloride in dimethylsulfoxide. The product and reaction intermediates were isolated, purified by preparative HPLC and analyzed in detail by infrared and nuclear magnetic resonance spectroscopy. The final product has a high cytotoxicity for L929 mouse fibroblasts, and low antibacterial activity against P. Aeruginosa and S. Aureus at concentrations of up to 20 mg/ml. It could potentially further be investigated for similar uses as suxamethonium chloride, a muscle relaxant drug. molekularna biologija holin klorid betain hidroklorid karbonildiimidazol protimikrobnost cititoksičnost choline chloride betaine hydrochloride carbonyldiimidazole HPLC antimicrobial cytotoxicity molekularna biologija holin klorid betain hidroklorid karbonildiimidazol protimikrobnost cititoksičnost true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/journal-of-molecular-structure © 2024 The Author(s) Članek v reviji 2024-08-09 05:47:44 2026-01-28 07:59:40 2026-01-29 03:11:44 0000-00-00 00:00:00 2024 0 0 [16] str. [article no.] 138581 Available online 8 May 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-01-22 2024-05-07 2024-05-08 577:576 195589379 10.1016/j.molstruc.2024.138581 1872-8014 1-s2.0-S0022286024011001-main.pdf 1-s2.0-S0022286024011001-main.pdf 1 BEE656A1B760959D4C34C99872E71D35 e91f7f7586a9190167ee6e07751c71c56f3b7ad9d7263aae0e62417e42129221 2e6f3a96-fc17-11f0-9a72-00155d00018d https://dk.um.si/Dokument.php?lang=slv&id=202659 https://www.sciencedirect.com/science/article/pii/S0022286024011001?via%3Dihub#sec0020 0 32b5ae25-fc17-11f0-9a72-00155d00018d https://dk.um.si/Dokument.php?lang=slv&id=202660 http://dx.doi.org/10.1016/j.molstruc.2024.138581 1 298df6e4-5602-11ef-97b6-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=182163 Fakulteta za strojništvo Fakulteta za kemijo in kemijsko tehnologijo 0 0 0