20.500.12556/DKUM-89869 Vpliv gnojenja z izrabljenim gobjim substratom na aktivnost talnih encimov v posameznih letnih časih magistrsko delo The impact of spent mushroom substrate fertilization on soil enzyme activity across different seasons Izrabljen substrat za gobe (IGS) je stranski proizvod gojenja gob, ki sestoji iz raznovrstnih organskih materialov. Po nekaj sezonah rabe se substrat izčrpa in praviloma velja za odpadek, vendar sodobne raziskave nakazujejo številne koristne možnosti uporabe izrabljenega gobjega substrata. V projektni raziskavi se je IGS uporabljal kot organsko gnojilo na treh različnih lokacijah v ekološki, integrirani in biodinamični pridelavi paradižnika v rastlinjakih. V vsaki od pridelav se je v triplikatih testiralo gnojenje z SMS, standardno gnojenje (S), SMS+S in kontrola brez gnojenja. V sklopu magistrskega dela smo na obravnavanih lokacijah jemali vzorce zemlje v vseh letnih časih, v vzorcih pa smo analizirali aktivnost encimov N-acetil-glukozaminidaza (NAG), fosfataza (PHOS), beta-glukozidaza (β0GLU), arilsulfataza (ARS), ureaza (URE), arilamidaza (ARN), alkalna fosfataza (PAK) in kisla fosfataza (PAC) po standardu ISO 20130:2018- Kakovost tal - Merjenje encimske aktivnosti v vzorcih tal s kolorimetričnimi substrati na mikrotitrski plošči. Primerjali smo razlike v encimski aktivnosti med posameznimi letnimi časi ter med različnimi načini gnojenja. Rezultati so pokazali, da različni letni časi vplivajo na aktivnost encimov v tleh, medtem ko dodatek gobjega substrata kot gnojila ne povzroči nujno povišanja encimske aktivnosti. Spent Mushroom Substrate (SMS) is a by-product of mushroom cultivation, consisting of various organic materials. After a few growing seasons, the substrate becomes depleted and is generally considered waste. However, modern research indicates numerous beneficial uses for spent mushroom substrate. In a project study, SMS was used as an organic fertilizer at three different locations in ecological, integrated, and biodynamic tomato production in greenhouses. In each cultivation method, fertilization with SMS, standard fertilization (S), SMS+S, and a control without fertilization were tested in triplicates. As part of the master's thesis, soil samples were taken from the observed locations in all seasons, and enzyme activities of N-acetyl-glucosaminidase (NAG), phosphatase (PHOS), beta-glucosidase (βGLU), arylsulfatase (ARS), urease (URE), arylamidase (ARN), alkaline phosphatase (PAK), and acid phosphatase (PAC) were analyzed in samples, according to the ISO 20130:2018 standard Soil Quality Measurement of enzyme activity patterns in soil samples using colorimetric substrates in microtiter plates. Differences in enzyme activities between the seasons and between different fertilization methods were compared. The results indicate, that different seasons affected enzyme activities in the soil, while the addition of mushroom substrate as a fertilizer did not necessarily result in an increased enzyme activity. Spent mushroom substrate (SMS) is a byproduct of mushroom cultivation, consisting of various organic materials. After several seasons of use, the substrate becomes depleted and is generally considered waste. However, modern research indicates numerous beneficial possibilities for the use of spent mushroom substrate. As part of the project EIP 16.5 "Cultivation of Mushrooms on the Farm and Its Role in the Circular Economy in the Preparation of Quality Organic Fertilizer," this type of spent mushroom substrate was used as organic fertilizer at three different locations in organic, integrated, and biodynamic farming. In the scope of the master's thesis, soil samples were taken at the mentioned locations in all seasons. The samples were analyzed for the activity of enzymes N-acetyl-glucosaminidase (NAG), phosphatase (PHOS), beta-glucosidase (0GLU), arylsulfatase (ARS), urease (URE), arylamidase (ARN), alkaline phosphatase (PAK), and acid phosphatase (PAC) according to the ISO 20130:2018 Standard - Soil Quality - Measurement of Enzymatic Activity in Soil Samples Using Colorimetric substrates in Micro-well Plates. We compared differences in enzyme activity between individual seasons and different fertilization methods. The results showed that different seasons affect soil enzyme activity, while the addition of mushroom substrate as fertilizer does not necessarily increase enzyme activity. talni encimi izrabljen gobji substrat aktivnost gnojenje kmetijstvo soil enzymes spent mushroom substrate activity fertilization agriculture true true false A. Štuhec Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-08-14 22:38:15 2024-09-03 10:49:35 2024-09-04 05:17:42 0000-00-00 00:00:00 2024 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (VIII, 97, [2] str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 631.46:631.8:635.8:631.86/.87(043)=163.6 206178051 MAG_Stuhec_Ana_2024.pdf MAG_Stuhec_Ana_2024.pdf 1 FB93A5535A5E30EEA528E053671705B4 aa2ddd4a02427e2aad49f672f369be3c68cb29b6fe3dc63b862439591de9b952 4e56dc0b-5ed2-11ef-abdc-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=182727 Fakulteta za kmetijstvo in biosistemske vede 0 0 0