20.500.12556/DKUM-90460-db4af0c4-7829-0f52-d352-ed74908c17c2 Mesocellular silica foam as immobilization carrier for production of statin precursors The employment of 2-deoxyribose-5-phosphate aldolase (DERA) stands as a prevalent biocatalytic route for synthesizing statin side chains. The main problem with this pathway is the low stability of the enzyme. In this study, mesocellular silica foam (MCF) with different pore sizes was used as a carrier for the covalent immobilization of DERA. Different functionalizing and activating agents were tested and kinetic modeling was subsequently performed. The use of succinic anhydride as an activating agent resulted in an enzyme hyperactivation of approx. 140%, and the stability almost doubled compared to that of the free enzyme. It was also shown that the pore size of MCF has a decisive influence on the stability of the DERA enzyme. immobilization aldolase DERA statins mesoporous silica pore size imobilizacija aldolaza statini mezoporozni silicijev dioksid velikost pore true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/ijms © 2024 by the authors Članek v reviji 2024-09-04 12:10:19 2024-09-04 12:10:20 2026-02-14 03:11:39 0000-00-00 00:00:00 2024 0 0 13 str. iss. 4, [article no.] 1971 Vol. 25 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0224-01-16 2024-02-03 2024-02-06 620.3 206319875 10.3390/ijms25041971 1422-0067 ijms-25-01971.pdf ijms-25-01971.pdf 1 DB64F0B3365DAA83B6EB1FA8C29A9A15 a1005abe94147d81bbf7238d0d2701129a73c8f483b17d55f1a827067d8557e4 819cb87a-08c6-11f1-8973-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203322 https://www.mdpi.com/1422-0067/25/4/1971 0 8b025bd2-08c6-11f1-8973-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203323 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0