20.500.12556/DKUM-90485-3f0eeff1-6d6d-4de3-89cd-4a0be6903e5e Ellagitannin content in extracts of the chestnut wood aesculus The chestnut tree (Castanea sativa Mill.) is a widespread plant in Europe, rich in high-value compounds, which can be divided mainly into monomeric polyphenols and tannins. These compounds exhibit various biological activities, such as antioxidant, as well as anticarcinogenic and antimicrobial properties. Chestnut wood (CW) extracts were prepared using different extraction techniques, process conditions, solvents, and their mixtures. This work aimed to test various extraction techniques and determine the optimal solvent for isolating enriched fractions of vescalagin, castalagin, vescalin, and castalin from CW residues. Supercritical CO2 extraction with a more polar cosolvent was applied at different pressures, which influenced solvent density. According to the results, the proportions of the components strongly depended on the solvent system used for the extraction. In addition, HPLC-DAD was used for semiqualitative purposes to detect vescalagin, castalagin, vescalin, and castalin. The developed valorization protocol allows efficient fractionation and recovery of the polyphenolic components of CW through a sustainable approach that also evaluates pre-industrial scaling-up. antioxidant activity chestnut wood conventional extraction extraction fractionation polyphenols tannins ultrasound extraction antioksidativno delovanje kostanjev gozd konvencionalna ekstrakcija ekstrakcija frakcioniranje polifenoli superkritična ekstrakcija tanini ultrazvočna ekstrakcija true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/molecules © 2024 by the authors Članek v reviji 2024-09-05 11:13:28 2024-09-05 11:13:29 2026-02-13 11:38:47 0000-00-00 00:00:00 2024 0 0 14 str. iss. 17, [article no.] 4015 Vol. 29 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-07-18 2024-08-22 2024-08-25 66.02 206458371 10.3390/molecules29174015 1420-3049 molecules-29-04015.pdf molecules-29-04015.pdf 1 3D25FD19D93FE1CD6D67AD62F7C1C38D 1d1e1fd094e80210d66dfe64e7925179684b75b97b203c47925532c9897d4bd6 d0270307-6b67-11ef-b541-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=184453 https://www.mdpi.com/1420-3049/29/17/4015 0 2cf0c238-08c8-11f1-8973-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203324 Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0