20.500.12556/DKUM-90528 Funkcionalizirana metalo-organska ogrodja za razgradnjo penicilina magistrsko delo Functionalized metallo-organic frameworks for penicillin degradation Onesnaževanje zaradi zlorabe antibiotikov resno ogroža okolje in zdravje ljudi, zato je nujno potreben razvoj učinkovitih strategij za razgradnjo in odstranjevanje ostankov antibiotikov. V magistrskem delu smo imobilizirali encim v metalo-organsko ogrodje (MOFs). V zeolitno imidazolatno ogrodje-8 (ZIF-8) smo z metodo samosestavljanja imobilizirali β-laktamazo (β-laktamaza@ZIF-8), katalitično učinkovitost imobiliziranih poroznih materialov (β-laktamaze@ZIF-8) za razgradnjo penicilinov (PEN) pa smo določevali z uporabo visoko zmogljivostne tekočinske kromatografije HPLC. Morfologijo in kemijsko strukturo β-laktamaze@ZIF-8 smo opredelili z vrstično elektronsko mikroskopijo (SEM). Rezultati so pokazali, da se je katalitična aktivnost enkapsuliranega encima v primerjavi s prostim encimom znatno povečala. Ugotovili smo tudi, da je ZIF-8 zaradi svoje velike površine in adsorpcijskih lastnosti že sam po sebi odlično orodje za odstranjevanje PEN iz vode. Katalitični mehanizem razgradnje PEN z β-laktamazo@ZIF-8 temelji na tvorbi kompleksa med ionom Zn (II) na ogrodju ZIF-8 in ciljno molekulo, kar oslabi vez štiričlenskega β-laktamskega obroča v molekuli PEN ter tako poveča učinkovitost encimske razgradnje PEN. Magistrsko delo je zagotovilo obetavno strategijo za razgradnjo antibiotika PEN v vodnem okolju. Pollution caused by the misuse of antibiotics poses a serious threat to the environment and human health, making the development of effective strategies for the degradation and removal of antibiotic residues urgently necessary. In this master's thesis, we immobilized an enzyme in a metal-organic framework (MOFs). Using a self-assembly method, we immobilized β-lactamase into a zeolitic imidazolate framework-8 (ZIF-8) (β-lactamase@ZIF-8), and the catalytic efficiency of the immobilized porous materials (β-lactamase@ZIF-8) for the degradation of penicillins (PEN) was determined using high-performance liquid chromatography (HPLC). The morphology and chemical structure of β-lactamase@ZIF-8 were characterized by scanning electron microscopy (SEM). The results showed that the catalytic activity of the encapsulated enzyme significantly increased compared to the free enzyme. We also found that due to its large surface area and adsorption properties, ZIF-8 is, in itself, an excellent tool for the removal of PEN from water. The catalytic mechanism of PEN degradation by β-lactamase@ZIF-8 is based on the formation of a complex between the Zn (II) ion in the ZIF-8 framework and the target molecule, which weakens the bond of the four-membered β-lactam ring in the PEN molecule, thus increasing the efficiency of enzymatic PEN degradation. This master's thesis provides a promising strategy for the degradation of the antibiotic PEN in aqueous environments. β-laktamaza kovinsko-organska ogrodja (MOFs) ZIF-8 penicilin (PEN) imobilizacija razgradnja Beta-lactamase metal-organic frameworks (MOFs) ZIF-8 penicillin (PEN) immobilization degradation. true true false [L. Dolinšek] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-09-06 19:36:07 2024-10-02 11:44:00 2024-10-19 03:10:52 0000-00-00 00:00:00 2024 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XII, 55 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.567:66.094.941(043.2) 212110851 MAG_Dolinsek_Lucija_2024.pdf MAG_Dolinsek_Lucija_2024.pdf 1 33A4C43F9C770BEEC8AF7E2222086D97 66eb009ce4f0f2c2a225ee79a3a37854d0e0076df410590e4fb654069198abb1 662cfe7f-801e-11ef-a73d-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186128 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0