20.500.12556/DKUM-90943 Adipose tissue gene expression of entire male, immunocastrated and surgically castrated pigs Differences in adipose tissue deposition and properties between pig male sex categories, i.e., entire males (EM), immunocastrates (IC) and surgical castrates (SC) are relatively well-characterized, whereas the underlying molecular mechanisms are still not fully understood. To gain knowledge about the genetic regulation of the differences in adipose tissue deposition, two different approaches were used: RNA-sequencing and candidate gene expression by quantitative PCR. A total of 83 differentially expressed genes were identified between EM and IC, 15 between IC and SC and 48 between EM and SC by RNA-sequencing of the subcutaneous adipose tissue. Comparing EM with IC or SC, upregulated genes related to extracellular matrix dynamics and adipogenesis, and downregulated genes involved in the control of lipid and carbohydrate metabolism were detected. Differential gene expression generally indicated high similarity between IC and SC as opposed to EM, except for several heat shock protein genes that were upregulated in EM and IC compared with SC. The candidate gene expression approach showed that genes involved in lipogenesis were downregulated in EM compared with IC pigs, further confirming RNA-sequencing results. pigs adipose tissue entire males immunocastration surgical castration RNA-sequencing expression prašiči maščobno tkivo samci imunokastracija kirurška kastracija sekvenciranje RNK izražanje true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2024-10-10 10:11:43 2024-10-10 10:35:58 2024-10-11 03:15:53 0000-00-00 00:00:00 2021 0 0 Str. 1-18 Št. 4, št. članka 1768 Letn. 22 10 Feb. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-01-11 2021-02-06 2021-02-10 636.4 50997507 10.3390/ijms22041768 1422-0067 Poklukar-2021-Adipose_Tissue_Gene_Expression_o.pdf Poklukar-2021-Adipose_Tissue_Gene_Expression_o.pdf 1 935E2B64F5108913AE3468CA2E951EF8 39be4343ec8f589b8fbfd4f3c10ba4b7f58de90eb81d5e51d708323164caee0b 5e137954-86e2-11ef-808f-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186372 https://doi.org/10.3390/ijms22041768 0 4a0dbd9e-86df-11ef-808f-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186369 Fakulteta za kmetijstvo in biosistemske vede 0 0 0