20.500.12556/DKUM-90981 Comparison of microbial populations in saliva and feces from healthy and celiac adolescents with conventional and molecular approaches after cultivation on gluten-containing media an exploratory study Microbes capable of metabolizing gluten are common in various parts of the intestinal tract. In this study, saliva and fecal samples were obtained from 10 adolescents (13-18 years of age), five of which had celiac disease (CD) and five of which were healthy volunteers (HV). Culture-enriched saliva and fecal samples were compared with molecular profiling, and microorganisms displaying lysis zones on gluten-containing media (i.e., gluten-degrading microorganisms; GDMs) were isolated. In total, 45 gluten-degrading strains were isolated, belonging to 13 genera and 15 species, including Candida albicans and Veillonella. GDMs were more common in HVs compared to CD patients and more diverse in saliva compared to feces. In saliva, GDMs showed partial overlap between HVs and CD patients. Bacterial communities in fecal samples determined with amplicon sequencing significantly differed between CD patients and HVs. Overall, 7-46 of all operational taxonomic units (OTUs) per sample were below the detection limit in the fecal samples but were present in the cultivated samples, and mainly included representatives from Lactobacillus and Enterococcus. Furthermore, differences in fecal short-chain fatty-acid concentrations between CD patients and HVs, as well as their correlations with bacterial taxa, were demonstrated. gluten-degrading microorganisms veillonella candida short-chain fatty acids celiac disease microbiota sequencing mikroorganizmi kandida kratkoverižne maščobne kisline celiakija mikrobiota sekvenciranje true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2024-10-14 12:57:37 2024-10-14 13:09:54 2024-10-15 03:10:59 0000-00-00 00:00:00 2021 0 0 Str. 1-9 št. 11, št. članka 2375 Letn. 9 Nov. 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-09-10 2021-11-10 2021-11-17 577 86065923 10.3390/microorganisms9112375 2076-2607 Senicar-2021-Comparison_of_Microbial_Populatio.pdf Senicar-2021-Comparison_of_Microbial_Populatio.pdf 1 44E30467EC0938ABE82E1C7DC66E1E65 af2bd184fdf03e7add985b4bb76bea2fea91e76899e5167142e729113afd38d7 b0c46584-8a1c-11ef-a9b1-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186473 https://doi.org/10.3390/microorganisms9112375 0 c2495a9b-8a1c-11ef-a9b1-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186474 Medicinska fakulteta 0 0 0