20.500.12556/DKUM-90985 Dual mode of action of acetylcholine on cytosolic calcium oscillations in pancreatic beta and acinar cells in situ Cholinergic innervation in the pancreas controls both the release of digestive enzymes to support the intestinal digestion and absorption, as well as insulin release to promote nutrient use in the cells of the body. The effects of muscarinic receptor stimulation are described in detail for endocrine beta cells and exocrine acinar cells separately. Here we describe morphological and functional criteria to separate these two cell types in situ in tissue slices and simultaneously measure their response to ACh stimulation on cytosolic Ca2+ oscillations [Ca2+]c in stimulatory glucose conditions. Our results show that both cell types respond to glucose directly in the concentration range compatible with the glucose transporters they express. The physiological ACh concentration increases the frequency of glucose stimulated [Ca2+]c oscillations in both cell types and synchronizes [Ca2+]c oscillations in acinar cells. The supraphysiological ACh concentration further increases the oscillation frequency on the level of individual beta cells, inhibits the synchronization between these cells, and abolishes oscillatory activity in acinar cells. We discuss possible mechanisms leading to the observed phenomena. pancreas tissue slices acetylcholine beta cell acinar cell Ca2+ oscillations tkivne rezine trebušne slinavke acetilholin beta celice acinarne celice kalcijeve oscilacije true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2024-10-14 14:44:13 2024-10-14 15:03:57 2024-10-15 03:10:59 0000-00-00 00:00:00 2021 0 0 Str. 1-14 št. 7, št. članka 1580 Letn. 10 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-05-14 2021-06-19 2021-06-23 612.349.7 68553731 10.3390/cells10071580 2073-4409 Sluga-2021-Dual_Mode_of_Action_of_Acetylcholin.pdf Sluga-2021-Dual_Mode_of_Action_of_Acetylcholin.pdf 1 F140052ACCE86C604686E7BCA11DAD5D 8e659ae7d595f0ed4284d9099053899d30525cacfc2aac4d4736346bedfb8dbd 9bf06b76-8a2c-11ef-a9b1-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186486 https://doi.org/10.3390/cells10071580 0 0a936d25-8a2a-11ef-a9b1-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186485 Medicinska fakulteta 0 0 0