20.500.12556/DKUM-91076 Investigating the viability of epithelial cells on polymer based thin-films The development of novel polymer-based materials opens up possibilities for several novel applications, such as advanced wound dressings, bioinks for 3D biofabrication, drug delivery systems, etc. The aim of this study was to evaluate the viability of vascular and intestinal epithelial cells on different polymers as a selection procedure for more advanced cell-polymer applications. In addition, possible correlations between increased cell viability and material properties were investigated. Twelve polymers were selected, and thin films were prepared by dissolution and spin coating on silicon wafers. The prepared thin films were structurally characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and goniometry. Their biocompatibility was determined using two epithelial cell lines (human umbilical vein endothelial cells and human intestinal epithelial cells), assessing the metabolic activity, cell density, and morphology. The tested cell lines showed different preferences regarding the culture substrate. No clear correlation was found between viability and individual substrate characteristics, suggesting that complex synergistic effects may play an important role in substrate design. These results show that a systematic approach is required to compare the biocompatibility of simple cell culture substrates as well as more complex applications (e.g., bioinks). HUIEC HUVEC morphology polymers thin films viability morfologija polimeri tanke plasti vitalnost true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2024-10-18 14:23:45 2024-10-18 14:34:28 2024-10-19 03:11:39 0000-00-00 00:00:00 2021 0 0 Str. 1-17 št. 14, št. članka 2311 Letn. 13 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-06-30 2021-07-09 2021-07-14 678 73616387 10.3390/polym13142311 2073-4360 Vihar-2021-Investigating_the_Viability_of_Epit.pdf Vihar-2021-Investigating_the_Viability_of_Epit.pdf 1 359220EFA8919F883BCA5FEA402A6F81 6ec0044e7773f9c313243853b4433119010ab2f362d92917879a46103adcd899 1a3458e3-8d4d-11ef-a8b7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186669 https://doi.org/10.3390/polym13142311 0 d281a763-8d4b-11ef-a8b7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=186668 Medicinska fakulteta 0 0 0