20.500.12556/DKUM-91359 Mesenchymal stem cells isolated from paediatric paravertebral adipose tissue show strong osteogenic potential Mesenchymal stem cells (MSCs) represent the basis of novel clinical concepts in cellular therapy and tissue regeneration. Therefore, the isolation of MSCs from various tissues has become an important endeavour for stem cell biobanking and the development of regenerative therapies. Paravertebral adipose tissue is readily exposed during spinal procedures in children and could be a viable source of stem cells for therapeutic applications. Here, we describe the first case of MSCs isolated from paravertebral adipose tissue (PV-ADMSCs), obtained during a routine spinal surgery on a child. Using quantitative real-time PCR and flow cytometry, we show that PV-ADMSCs have different levels of stem marker expression compared to the MSCs from other sources while having the highest proliferation rate. Furthermore, we evaluate the multipotency of PV-ADMSCs by the three-lineage (adipogenic, osteogenic and chondrogenic) differentiation and compare it to the multipotency of MSCs from other sources. It was found that the PV-ADMSCs have a strong osteogenic potential in particular. Taken together, our data indicate that PV-ADMSCs meet the criteria for successful cell therapy, defined by the International Society for Cellular Therapy (ISCT), and thus, could provide a source of MSCs that is relatively easy to isolate and expand in culture. Due to their strong osteogenic potential, these cells provide a promising basis, especially for orthopaedic applications. mesenchymal stem cells paediatric surgery paravertebral adipose tissue regenerative medicine stem marker expression osteogenic potential differentiation mezenhimske matične celice otroška kirurgija paravertebralno maščobno tkivo regenerativna medicina izražanje matičnih označevalcev osteogeni potencial diferenciacija true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2024-12-16 08:56:47 2024-12-16 09:20:00 2024-12-17 03:11:09 0000-00-00 00:00:00 2022 0 0 Str. 1-18 št. 2, št. članka 378 Letn. 10 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-12-10 2022-02-02 2022-02-04 617.3 96502787 10.3390/biomedicines10020378 2227-9059 Rozanc_2022_Mesenchymal_Stem_Cells_Isolated.pdf Rozanc_2022_Mesenchymal_Stem_Cells_Isolated.pdf 1 245E6F7717191280212634706E3CA5EC 7eea112596e8454e75f831353fa8190184ad5325505bff4ff59d8572f6db73f7 f7a0c4f6-bb85-11ef-8250-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=187365 https://doi.org/10.3390/biomedicines10020378 0 4db0748e-bb83-11ef-8250-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=187364 Medicinska fakulteta 0 0 0