20.500.12556/DKUM-91628 Hydroxymethylfurfural affects caged honey bees (Apis mellifera carnica) A high concentration of hydroxymethylfurfural (HMF) (e.g., 15 mg HMF per kg honey) indicates quality deterioration for a wide range of foods. In honey bee colonies, HMF in stored honey can negatively affect bee health and survival. Therefore, in the laboratory, we experimentally determined the effects of HMF on the longevity and midgut integrity of worker Apis mellifera carnica by feeding bees standard diets containing five concentrations of HMF (100, 500, 1000, and 1500 ppm). Simultaneously, we also examined HMF%s effect on Nosema ceranae spore counts within infected honey bees. We performed an immunohistochemical analysis of the honey bee midgut to determine possible changes at the cellular level. No correlation was established between HMF concentration and N. ceranae spore counts. Negative effects of HMF on bees were not observed in the first 15 days of exposure. However, after 15 to 30 days of exposure, HMF caused midgut cells to die and an increased mortality of honey bee workers across treatment groups. hydroxymethylfurfural honey bee cell death immunohistochemistry Nosema ceranae hidroksimetilfurfural medonosne čebele celična smrt imunohistokemija true false true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2025-01-21 14:39:11 2025-01-21 14:52:26 2025-01-22 03:11:11 0000-00-00 00:00:00 2020 0 0 Str. 1-10 št. 1, št. članka 18 Letn. 12 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2019-11-28 2019-12-28 2019-12-31 638.1 4646956 10.3390/d12010018 1424-2818 Gregorc-2020-Hydroxymethylfurfural_Affects_Cag.pdf Gregorc-2020-Hydroxymethylfurfural_Affects_Cag.pdf 1 34B0FA8856AEFB1C901BEFDA10835363 93e5712bc89d5054c39fdab1fb61fe6cc1feccd680659717e85a92d979115b9e d32c5900-d7fe-11ef-b93e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188022 https://doi.org/10.3390/d12010018 0 d9be2283-d7fe-11ef-b93e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188023 Fakulteta za kmetijstvo in biosistemske vede 0 0 0