20.500.12556/DKUM-91659 Development of Biomimetic Sensors to Determine the Functionality of in Vitro Cell Models Razvoj biomimetičnih senzorjev za določanje funkcionalnosti in vitro celičnih modelov Advances in biomedical science have enabled the development of in vitro models that mimic human tissues. Non-invasive real-time monitoring of these models would provide valuable insights without disrupting the cellular environment. This study explores the use of molecularly imprinted polymers (MIPs) for this purpose, focusing on insulin and lactate as biomarkers of cellular metabolism. MIP sensors were created by electropolymerizing pyrrole around insulin or lactate templates on carbon electrodes. The sensors showed high sensitivity and selectivity, with detection ranges of 20.0–70.0 pM for insulin (LOD: 2.41 pM) and 0.5–3.0 mM for lactate. These findings highlight MIPs’ potential for personalized diagnostics and therapy monitoring. Napredek v biomedicinskih znanostih je omogočil razvoj in vitro modelov, ki posnemajo človeška tkiva. Neinvazivno spremljanje teh modelov v realnem času bi omogočilo dragocene vpoglede brez motenja celičnega okolja. Ta študija raziskuje uporabo molekularno vtisnjenih polimerov (MIPs) za ta namen, s poudarkom na inzulinu in laktatu kot biomarkerjih celičnega metabolizma. Senzorji MIP so bili ustvarjeni z elektropolimerizacijo pirrola okoli predloge inzulina ali laktata na karbonskih elektrodah. Senzorji so pokazali visoko občutljivost in selektivnost z razponi zaznave od 20,0 do 70,0 pM za inzulin (LOZ: 2,41 pM) in od 0,5 do 3,0 mM za laktat. Ti rezultati poudarjajo potencial MIP za uporabo v personalizirani diagnostiki in spremljanju terapij. biomimetic sensors molecular imprinting polypyrrole matrix insulin MIP sensor biomimetični senzorji molekularno vtiskanje polipirol inzulin MIP senzor true false true Univerza v Mariboru, Univerzitetna založba Angleški jezik Slovenski jezik Znanstveno delo 2025-01-24 11:04:53 2025-01-24 11:14:10 2026-04-09 16:03:42 0000-00-00 00:00:00 2024 0 Maribor 0 19 št.2 Letn.14 Dec. 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-12-20 2024-10-09 2024-12-23 60 223684355 10.18690/analipazu.14.2.39-57.2024 2820-364X Development_of_biomimetic_sensors-Frangez-2024.pdf Development_of_biomimetic_sensors-Frangez-2024.pdf 1 0DC348A3A1577E71B5D86F77CBA26788 dbf5879976c79deead611838411e1a418f519a1d2c9a2bbe454d0b5e84ecf94b 88f6a52c-da3b-11ef-9445-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188123 https://journals.um.si/index.php/anali-pazu/article/view/4803 1 a9e744bb-da3a-11ef-9445-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188122 Medicinska fakulteta Univerzitetna založba Univerze v Mariboru Anali PAZU 0 0 0