20.500.12556/DKUM-91674 Clostridioides difficile ribotype distribution in a large teaching hospital in Serbia Background: The global epidemic of nosocomial diarrhea caused by Clostridioides (Clostridium) difficile started in 2000, with high mortality rates and emergence of a new hypervirulent strain NAP1/BI/027. The aim of this study was to assess the presence of ribotype 027 and other C. difficile ribotypes in a Serbian University Hospital, compare the temporal variability of ribotypes 3 years apart, as well as to compare clinical, demographic and laboratory characteristics and disease outcome among patients infected with 027 and non-027 ribotype. This was a prospective observational cohort study addressing 4-month intervals during 2014/2015 and 2017/2018. Results: Ribotyping was performed in 64 non-duplicate C. difficile strains. Ribotype 027 was the most prevalent, and was detected in 53 (82.8%) patients (43/45 and 10/19 patients in 2014-2015 and 2017/2018, respectively). Other detected ribotypes were 001/072 in 4 (6.3%), 002 in 4 (6.3%), 014/020 in 2 (3.1%) and 176 in 1 (1.5%) patient. The percentage of the patients infected with ribotype 027 significantly decreased during the 3-year period, from 95.6 to 52.6% (p < 0.001). Ribotype 027 infection was associated with fluoroquinolone treatment more frequently than infection with other ribotypes [33 (62.3%) vs. 2 (18.2%), p = 0.010)]. A severe C. difficile infection was diagnosed more often in patients with the detected ribotype 027 compared to those infected with non-027 ribotypes (p = 0.006). No significant difference in the mortality and recurrence rates was found between the patients infected with ribotype 027 and those infected with other ribotypes [10/53 (18.8%) vs. 2/11 (18.2%), p = 0.708, and 10/35 (28.6%) vs. 0/2 (0%), p = 1.000, respectively]. Conclusion: Clostridium difficile ribotype 027 was the most prevalent ribotype among patients in a large Serbian hospital, but there is a clear decreasing trend. Clostridium difficile ribotype 027 Serbia ribotip 027 Srbija true false true BioMed Central Angleški jezik Slovenski jezik Znanstveno delo 2025-01-27 09:17:02 2025-01-27 09:56:40 2025-01-28 03:09:18 0000-00-00 00:00:00 2020 0 0 Str. 1-6 št. članka 26 Letn. 12 May 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2020-01-24 2020-05-16 2020-05-22 579.852.13 26265603 10.1186/s13099-020-00364-7 1757-4749 Korac-2020-_i_Clostridioides_difficile__i__rib.pdf Korac-2020-_i_Clostridioides_difficile__i__rib.pdf 1 D584D96CBDD41FEC24B074E4C0574F61 3969118a80e5a880a95370fe8bae88000d773a235ec6c482b2b097812795dc7e 6bd37f11-dc8a-11ef-9f3b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188161 https://doi.org/10.1186/s13099-020-00364-7 0 170fca0e-dc87-11ef-9f3b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188159 Medicinska fakulteta 0 0 0