20.500.12556/DKUM-91707 Microbiota in vitro modulated with polyphenols shows decreased colonization resistance against Clostridioides difficile but can neutralize cytotoxicity While the knowledge on gut microbiota - C. difficile interactions has improved over the years, the understanding of the underlying mechanisms providing colonization resistance as well as preventative measures against the infection remain incomplete. In this study the antibiotic clindamycin and polyphenol extracts from pomegranate and blueberries were used individually and in combination to modulate fecal microbial communities in minibioreactor arrays (MBRA). Modulated communities were inoculated with C. difficile (ribotype 027). Subsequent 7-day periodical monitoring included evaluation of C. difficile growth and activity of toxins TcdA and TcdB as well as analysis of MBRA bacterial community structure (V3V4 16%S metagenomics). Polyphenols affected multiple commensal bacterial groups and showed different synergistic and antagonistic effects in combination with clindamycin. Exposure to either clindamycin or polyphenols led to the loss of colonization resistance against C. difficile. The successful growth of C. difficile was most significantly correlated with the decrease in Collinsella and Lachnospiraceae. Additionally, we demonstrated that Clostridium sporogenes decreased the activity of both C. difficile toxins TcdA and TcdB. The feature was shown to be common among distinct C. sporogenes strains and could potentially be applicable as a non-antibiotic agent for the alleviation of C. difficile infection. microbiota in vitro Clostridioides difficile resistance cytotoxicity mikrobiota C. difficile odpornost citotoksičnost true false true Nature Publishing Group Angleški jezik Slovenski jezik Znanstveno delo 2025-01-29 14:34:17 2025-01-29 14:47:12 2025-01-30 03:11:22 0000-00-00 00:00:00 2020 0 0 Str. 1-11 št. 8358 Letn. 10 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2020-01-13 2020-03-21 2020-05-20 579.852.13 16205827 10.1038/s41598-020-65253-0 2045-2322 Mahnic-2020-Microbiota__i_in_vitro__i__modulat.pdf Mahnic-2020-Microbiota__i_in_vitro__i__modulat.pdf 1 2CC00F4AB632A741987E0AB2C703F5C0 bb6f6c4d2e5bb6c57f4f5f7c649fe1239dd15de3a594fc1d949e64ada7881acb 60624cd5-de47-11ef-9dfa-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188284 https://doi.org/10.1038/s41598-020-65253-0 0 be07a1e6-de45-11ef-9dfa-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188283 Medicinska fakulteta 0 0 0