20.500.12556/DKUM-91792 Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell lines Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated. Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions. Conclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis. Campylobacter jejuni Lactobacillus spp. chicken and pig cell line adhesion invasion translocation probiotične bakterije Lactobacillus sp. učinkovitost probiotičnih bakterij adhezivnost invazivnost Campylobacter jejuni celične kulture črevesne epitelijske celice translokacija true false true BioMed Central Angleški jezik Slovenski jezik Znanstveno delo 2025-02-06 13:09:11 2025-02-06 13:34:13 2025-02-07 03:11:39 0000-00-00 00:00:00 2020 0 0 Str. 1-14 št. članka 34 Letn. 16 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2019-09-03 2020-01-09 2020-02-03 579.22+579.24:636.4/.5.09 512948536 10.1186/s12917-020-2238-5 1746-6148 Pogacar-2020-Effect_of__i_Lactobacillus__i__sp.pdf Pogacar-2020-Effect_of__i_Lactobacillus__i__sp.pdf 1 58DAC805280E867DE434D4D0070ED99B 01814000c36923a2545e841557aa17e8cce4000fdbd21563c9aba9772552a1a0 43fc409a-e486-11ef-8133-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188490 https://doi.org/10.1186/s12917-020-2238-5 0 2d100a3e-e483-11ef-8133-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188489 Medicinska fakulteta Fakulteta za kmetijstvo in biosistemske vede 0 0 0