20.500.12556/DKUM-91846 An overview of the current state of cell viability assessment methods using OECD classification Over the past century, numerous methods for assessing cell viability have been developed, and there are many different ways to categorize these methods accordingly. We have chosen to use the Organisation for Economic Co-operation and Development (OECD) classification due to its regulatory importance. The OECD categorizes these methods into four groups: non-invasive cell structure damage, invasive cell structure damage, cell growth, and cellular metabolism. Despite the variety of cell viability methods available, they can all be categorized within these four groups, except for two novel methods based on the cell membrane potential, which we added to the list. Each method operates on different principles and has its own advantages and disadvantages, making it essential for researchers to choose the method that best fits their experimental design. This review aims to assist researchers in making this decision by describing these methods regarding their potential use and providing direct references to the cell viability assessment methods. Additionally, we use the OECD classification to facilitate potential regulatory use and to highlight the need for adding a new category to their list. cell viability cell-based methods in vitro toxicology OECD cell viability classification true true true MDPI Angleški jezik Angleški jezik Znanstveno delo 2025-02-13 12:13:55 2025-02-13 12:21:46 2025-02-14 03:12:50 0000-00-00 00:00:00 2025 0 0 Str. 1-18 Št. 1, št. članka 220 Letn. 26 Jan. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-12-27 2024-12-29 2024-12-30 576 225295363 10.3390/ijms26010220 1422-0067 RAZ_Madorran_Eneko_2025.pdf RAZ_Madorran_Eneko_2025.pdf 1 AF194C1F67EEFCB47BB756B395CC01E0 4a35724ed5cfa287a3d02819469c28606df6ed8e149dc5f514ebca5124cade55 a089b21d-e9fc-11ef-ae38-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188640 https://doi.org/10.3390/ijms26010220 1 9e81d943-e9fb-11ef-ae38-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188638 https://www.mdpi.com/1422-0067/26/1/220 1 9eda0817-e9fb-11ef-ae38-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=188639 Medicinska fakulteta 0 0 0