20.500.12556/DKUM-92051 Development of fluorescence-based method for dopamine determination using o-phthaldialdehyde and 3-mercaptopropyltriethoxysilane Nanomaterials and sensors play an important role in modern technologies, including medical diagnostics and biochemical research. This work presents the possibility of using o-Phthaldialdehyde (OPA) in combination with 3-mercaptopropyltriethoxysilane (MPTES) to develop a dopamine-responsive sensor. During the experiment, these materials were used at different pH and ratios to determine the optimal parameters for obtaining high fluorescence intensity of the reaction product. The data obtained demonstrate a linear relationship between the fluorescence response (λex/λem = 340/460 nm) of OPA/MPTES and dopamine concentration in the range of 0.1–3.0 µM at a pH of 8, and the detection limit was 8.7 nM. The obtained results confirm the potential of OPA/MPTES as a sensing component for the detection of dopamine fluorometric method dopamine o-phthalaldehyde dopamini true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/sensors © 2025 by the authors Članek v reviji 2025-03-13 11:11:10 2025-03-13 11:11:10 2026-01-08 14:04:58 0000-00-00 00:00:00 2025 0 0 14 str. iss. 6, [article no.] 1729 Vol. 25 7 Mar. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-01-30 2025-03-07 2025-03-11 66 228846339 10.3390/s25061729 1424-8220 sensors-25-01729.pdf sensors-25-01729.pdf 1 8C114D613CCBA920A8A46F79C9DE924C eff95ac0a3b25b0ca1540996b1c3b1c41ac4eb4a11fb21c0e17ba5baaa5d78f9 d20f9403-fff3-11ef-82d0-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189242 https://www.mdpi.com/1424-8220/25/6/1729 0 a1ce66ab-ec92-11f0-a4e9-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201839 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0