20.500.12556/DKUM-92101 Copper sulfate elicitation effect on biomass production, phenolic compounds accumulation, and antioxidant activity of Morus nigra L. stem node culture Phenolic compounds are strong antioxidant and antibacterial agents with great pharmacological, medicinal, nutritional, and industrial value. The potential of Morus nigra in stem node culture was investigated for the production of phenolic compounds and their elicitation with CuSO4. Individual phenolic compounds in the samples were identified and quantified by using HPLC-PDA and HPLC-MS methods, while the content of total phenolic compounds, the content of total flavonoids, and the antioxidant activity of methanolic extracts were evaluated spectrophotometrically. The highest fresh and dry weights were obtained in plantlets treated with 0.5 mM CuSO4 for 42 days. The highest total phenolic content, total flavonoid content, and antioxidant activity of the extracts were determined in stem node cultures treated with 3 mM CuSO4 for 42 days. Under the latter conditions, the predominant representatives of the caffeoylquinic acids, p-coumaric acid derivatives, kaempferol derivatives, and quercetin derivatives also achieved the highest content. The most abundant phenolic compound in all samples was the chlorogenic acid. The nodal culture of M. nigra elicited with CuSO4 could potentially be used for the industrial production of phenolic compounds, especially caffeoylquinic acids. Moreover, considering the biochemical response to CuSO4 treatment and the ability to tolerate and accumulate copper, the potential application of M. nigra in phytoremediation is also highlighted. antioxidative potential black mulberry copper stress elicitation of phenolics flavonoids in vitro culture nodal culture phenolic acids true true true Angleški jezik Angleški jezik Znanstveno delo 2025-03-14 03:23:22 2025-03-25 09:41:54 2025-10-07 10:58:22 0000-00-00 00:00:00 2025 0 0 38 str. Iss. 5, [article no.] 766 Vol. 14 2025 0000-00-00 Zaloznikova Objavljeno apc 2025-02-22 2025-02-28 2025-03-02 581 228015363 10.3390/plants14050766 2223-7747 https://www.mdpi.com/2223-7747/14/5/766 1 4d602bd6-007b-11f0-9c63-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189309 Senekovic-2025-Plants_Cooper.pdf Senekovic-2025-Plants_Cooper.pdf 1 4A433F723C382BC431749DBDD185FEE2 ff0ff4f78cb05cdeee2788e70a190bc622e6cb00b2c46bf1c2108f1268cd921d c9ce6834-7e8c-11f0-9610-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=195860 Fakulteta za kmetijstvo in biosistemske vede 0 0 0