20.500.12556/DKUM-92180 Keratinski nanodelci iz produkta hidrotermične razgradnje perja/volne in formulacija premazov na celulozne tekstilije magistrsko delo Keratin nanoparticles from the hydrothermal degradation product of feathers/wool and formulation of coatings on cellulosic textiles V magisterskem delu je predstavljena sinteza delcev iz ekstrahiranega keratina iz odpadne volne oz. perja in kvaterniziranega hitozana. Cilj je, da dobimo nanodelce (velikost delcev do 100 nm), katere bi uporabili za medicinske tekstilije namenjene celjenju ran. Keratinske suspenzije smo še dodatno prečistili z dializo in filtrirali skozi 1 μm filter. Sintezo nanodelcev smo izvedli z metodo ionskega geliranja, kjer smo počasi dodajali hitozan v keratin pri pH=5, konstantnem mešanju in pri sobni tempraturi z namenom, da bi zamrežili keratinske karboksilne (negativne) skupine s hitozanskimi aminskimi (pozitivnimi) skupinami. Pri optimizaciji razmerij in sprotnem merjenju zeta potenciala je bila izbrana mešanica F v množinskem razmerju hitozan:keratin=1:5000. Ta je pri meritvah zeta potenciala skozi pH območje med 2 in 9, imela izoelektrično točko pri pH okoli 7 in s tem obdržala tako pozitiven kot negativen značaj, kar kaže na amfoterni značaj in uspešno zamreženje. S tem v delce doprinesemo antioksidativne kot protimikrobne značilnosti obeh združenih polimerov. Optimalni mešanici smo nanesli na bombažno tekstilijo in preverili antioksidativnost le te, ki je bila visoka. Nazadnje smo naredili test omočljivost tekstilije in izmerili ATR-FTIR spekter, s pomočjo katerega smo ugotovili, da pri sintezi premaza in premazovanju ni prišlo do spremembe glavnih funkcionalnih skupin, The master's thesis presents the synthesis of particles from extracted keratin derived from waste wool or feathers and quaternized chitosan. The goal is to obtain nanoparticles (particle size up to 100 nm) that can be used for medical textiles intended for wound healing. Keratin suspensions were further purified by dialysis and filtered through a 1 μm filter. The synthesis of nanoparticles was carried out using the ionic gelation method, where chitosan was slowly added to keratin at pH = 5, under constant stirring and at room temperature, aiming to crosslink keratin’s carboxyl (negative) groups with chitosan’s amine (positive) groups. During the optimization of ratios and continuous zeta potential measurements, mixture F was selected in a molar ratio of chitosan:keratin = 1:5000. This mixture exhibited an isoelectric point at approximately pH 7 when measured across the pH range of 2 to 9, maintaining both positive and negative charges, indicating an amphoteric nature and successful crosslinking. This contributes to the particles' antioxidant and antimicrobial properties, attributed to the combined polymers. The optimal mixture was applied to cotton textiles, and its antioxidant activity was evaluated, showing high effectiveness. Finally, a wettability test was performed on the textile, and an ATR-FTIR spectrum was measured, confirming that the synthesis and coating process did not alter the main functional groups characteristic of keratin and chitosan. ionsko geliranje keratin hitozan nanodelci celjenje ran antioksidativnost ionic gelation keratin chitozan nanoparticles wound healing antioxidant activity true true false [T. Gavrić] Slovenski jezik Angleški jezik Magistrsko delo 2025-03-19 07:28:49 2025-04-02 15:10:19 2025-04-26 03:09:37 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (X, 54 str.)) 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 547.962.9:[604.4:615.33](043.2) 234292227 MAG_Gavric_Tamara_2025.pdf MAG_Gavric_Tamara_2025.pdf 1 8B6FDDEBCCDDE69D69161B6436C48C8F e0fa807100712b41987da124bdf109b9dc0d2ebf7168ccb96d3f8826ffa67311 967e195e-0fc3-11f0-bfc8-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189942 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0