20.500.12556/DKUM-92182 Functionalization of polycaprolactone 3D scaffolds with hyaluronic acid glycine-peptide conjugates and endothelial cell adhesion This study enhances the bioactivity of polycaprolactone (PCL) scaffolds for tissue engineering by functionalizing them with oxidized hyaluronic acid glycine-peptide conjugates to improve endothelial cell adhesion and growth. Hyaluronic acid was conjugated with a glycine-peptide to create a bioactive interface on PCL (static water contact angle, SCA(H2O): 98°). The scaffolds were fabricated using a melt extrusion 3D printing technique. The HA-glycine peptide conjugates were oxidized and immobilized on aminolyzed PCL via Schiff-base chemistry, introducing hydrophilicity (SCA(H2O): 21°), multiple functional groups, and a negative zeta potential (-12.04 mV at pH 7.4). A quartz crystal microbalance confirmed chemical conjugation and quantified the mass (8.5-10.3 mg m-2) of oxidized HA-glycine on PCL. The functionalized scaffolds showed enhanced swelling, improved mechanical properties (2-fold increase in strength, from 26 to 51 MPa), and maintained integrity during degradation. In-vitro experiments demonstrated improved endothelial cell adhesion, proliferation and viability, suggesting the potential for vascularized tissue constructs. 3D printing polycaprolactone hyaluronic acid true true true ACS Publications Angleški jezik Angleški jezik Znanstveno delo 2025-03-19 08:32:27 2025-03-19 08:44:32 2025-03-20 03:10:47 0000-00-00 00:00:00 2025 0 0 Str. 1771−1787 Št. 3 Letn. 26 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-11-08 2025-02-11 2025-02-24 604 228392451 10.1021/acs.biomac.4c01559 1526-4602 RAZ_Mohan_Tamilselvan_2025.pdf RAZ_Mohan_Tamilselvan_2025.pdf 1 B75ADFE46129659CF0596EAB273BCF0D 4d9c73718d82aa0301c93bff39ba5e48c21065106a2629c5a4bf277f4a9020b9 58c1a730-0495-11f0-a5c9-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189465 https://pubs.acs.org/doi/full/10.1021/acs.biomac.4c01559 1 50529327-0494-11f0-a5c9-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189464 Medicinska fakulteta 0 0 0