20.500.12556/DKUM-92192 Comparison of conventional and supercritical isolation methods from bee pollen and their effects on the concentrations of bioactive compounds The present work represents a profound investigation of material pretreatment, comparison of different isolation methods (conventional and supercritical), and characterization of phenolic compounds and amino acids from bee pollen. The determination of amino acids was carried out after developing a novel hydrophilic interaction chromatography combined with quadrupole time-of-flight tandem mass spectrometric method (HILIC-QTOF-MS/MS) with limits of detection over the range 1 mg/kg -5 mg/kg and recoveries over the range 87.6% - 97.1%. The optimal conditions for the isolation of amino acids are in the range of temperatures between 25°C to 55°C and at pressures between 300 bar and 350 bar. It can be concluded that the extraction temperature and the pressure affect antioxidant activity. The favored conditions are lower temperatures and higher pressures. bee pollen in vitro antioxidant activity HILIC-LC-QTOF-MS.MS amino acids WM-266-4 cells čebelji cvetni prah amino kisline true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/the-journal-of-supercritical-fluids © 2025 The Author(s) Članek v reviji 2025-03-20 08:18:18 2025-03-20 08:18:18 2026-01-13 03:17:14 0000-00-00 00:00:00 2025 0 0 34 str. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-10-18 2025-03-14 2025-03-18 66.02 229553667 10.1016/j.supflu.2025.106599 1872-8162 1-s2.0-S0896844625000865-main.pdf 1-s2.0-S0896844625000865-main.pdf 1 AAB80705C77E0C8578527BB30EF7A2E3 f626a1f30b94b880b58e5105da677955be5df8f8cddb590e7d3e1c0970f77ac6 5a324d18-ef8f-11f0-8437-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201901 https://www.sciencedirect.com/science/article/pii/S0896844625000865?via%3Dihub 0 f38fe65d-ef8d-11f0-8437-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201900 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0