20.500.12556/DKUM-92200 Study of a sensitive and selective electrochemical biosensor for glucose based on ▫$Bi_2Ru_2O_7$▫ pyrochlore clusters combined with MWCNTs The development of sensitive, selective, and reliable glucose biosensors remains a persistent challenge in clinical diagnostics. In this study, we exploited the advantageous (electro)catalytic properties of bismuth ruthenate (Bi2Ru2O7) pyrochlore clusters, known for their high surface activity and metallic-like conductivity, and the favorable physicochemical properties of multi-walled carbon nanotubes (MWCNTs) by combining them with glucose oxidase (GOD) in a sensitive and selective disposable glucose biosensor. The integration of Bi2Ru2O7 enabled an enhanced and more reproducible response of the biosensor along with fast and improved communication between the supporting electrode and the upper biosensing layer. The architecture of the biosensor involves the deposition of an MWCNT layer on a ferrocyanide-modified screen-printed carbon electrode (FCN-SPCE), followed by the application of a biorecognition layer including GOD and Bi2Ru2O7 clusters. The voltammetric biosensor showed excellent electroanalytical performance, capable of detecting low glucose concentrations with a detection limit of 40 μM along with a linear response across the examined concentration range of 1.0–20.0 mM. The biosensor exhibited good reproducibility with a relative standard deviation (RSD) of 1.2% and interference-free operation against several of the most common interfering compounds. The practical applicability of the biosensor was demonstrated by the determination of glucose in a real serum sample spiked with different concentrations of glucose. glucose sensor glucose oxidase Bi2Ru2O7 pyrochlore multi-walled carbon nanotubes voltammetry serum senzorji glukoze oksidaza glukoze voltametrija true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/chemosensors © 2025 by the authors Članek v reviji 2025-03-20 11:35:02 2025-03-20 11:35:02 2026-01-10 03:08:15 0000-00-00 00:00:00 2025 0 0 13 str. iss. 3, [article no.] 109 Vol. 13 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-01-14 2025-03-13 2025-03-15 544 229474563 10.3390/chemosensors13030109 2227-9040 chemosensors-13-00109-v2.pdf chemosensors-13-00109-v2.pdf 1 B65D1618A5E8F105DE8CDD1E3E1F421B 844d7c5b707f45406006a80c03095076ac1406fe372715fbc1bb09be1ec99ee7 b491c8a9-ed4f-11f0-8729-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201867 https://www.mdpi.com/2227-9040/13/3/109 0 86cb5a25-ed4f-11f0-8729-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201866 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0