20.500.12556/DKUM-92218 Harnessing environmental yeasts - Pichia kudriavzevii strain ZMUM_K002 the quest for isolates with properties for efficient biotechnological applications The environment hosts a diversity of microorganisms whose potential for biotechnological applications has not yet been exhausted. The quest of our study was to find isolates of Pichia kudriavzevii from the environment that could be used as new biotechnological agents. Moreover, we aimed to explore the resource efficiency for microbial cultivation, in particular the efficiency of spent coffee grounds (SCG), an easily accessible waste coffee product with a high unutilized organic content. In this study, Pichia kudriavzevii strain ZMUM_K002, a yeast strain isolated from a grape pomace compost, was investigated. Antifungal susceptibility, particularly fluconazole susceptibility, was assessed, and the strain’s biotechnological potential by comparing its ability to utilize low-cost carbon sources, including SCG, with a natural isolate of Saccharomyces cerevisiae (strain ZMUM_K003) was assessed. The P. kudriavzevii strain ZMUM_K002 exhibited higher fluconazole susceptibility and yielded more than 30% more biomass in optimized media formulations compared to S. cerevisiae ZMUM_K003. These findings demonstrate that P. kudriavzevii ZMUM_K002 has the potential for efficient biomass production in sustainable industrial biotechnology, particularly in processes requiring high biomass yields on alternative substrates. Pichia kudriavzevii Candida krusei environmental sampling biomass production sauerkraut safety assessment spent coffee grounds vzorčenje okolja proizvodnja biomase ocena varnosti porabljena kavna usedlina true false true Angleški jezik Slovenski jezik Znanstveno delo 2025-03-21 09:22:10 2025-03-21 09:22:10 2025-03-22 03:07:51 0000-00-00 00:00:00 2025 0 0 15 str. št. 1, št. članka 30 Letn. 5 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-01-30 2025-03-11 2025-03-13 582.683.211:613.292:663.15 229771523 10.3390/applmicrobiol5010030 2673-8007 applmicrobiol-05-00030.pdf applmicrobiol-05-00030.pdf 1 E85A3A1A3B9D777F6362061FCAD0D707 af6d862e5c7fa5cbdafc05a0a8b58382738fdf23c06dcb698cbb2b0218a6b90c f75b6797-062d-11f0-90ca-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189549 https://doi.org/10.3390/applmicrobiol5010030 1 966c760b-062d-11f0-90ca-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189548 Fakulteta za naravoslovje in matematiko 0 0 0