20.500.12556/DKUM-92245 Unraveling the antibiofilm activity of a new nanogold resin for dentures and epithesis Dentures and epitheses are mostly made from poly(methyl methacrylate) (PMMA), which does not show antimicrobial properties. They present reservoirs of microorganisms grown in biofilms. The aim of this study is to prepare a PMMA enriched with gold nanoparticles (AuNPs)- PMMA/AuNPs and the examination of its physical, mechanical and antimicrobial properties. The AuNPS were synthetized from HAuCl4 using the ultrasonic spray pyrolysis method with lyophilization. The PMMA/AuNP samples were compared to PMMA samples. Density was measured by pycnometer. Microhardness was evaluated using the Vickers hardness test. Monomicrobial biofilm formation (Streptococcus mitis, Candida albicans, Staphylococcus aureus and Escherichia coli) was measured by colony-forming units (CFUs) and MTT test and visualized by SEM. AuNP release was measured indirectly (the CFUs of the medium around the sample). The density and microhardness of the PMMA/AuNPs were similar to those of the PMMA. CFU and MTT values for the biofilms formed on the PMMA for each of the tested species were higher than those of the biofilms formed on the PMMA/AuNPs. The CFUs of the medium around the sample were similar for both materials. PMMA/AuNPs showed a significant reduction in the monomicrobial biofilms of all tested species. AuNPs are not released from PMMA/AuNPs. Density, indirect measurement of residual monomer and dentures weight were similar between PMMA and PMMA/AuNPs. Microhardness, as a measure of the wear resistance, was also similar between tested discs. PMMA gold nanoparticles (AuNPs) biofilm antibiofilm effect metalurgija zlati nanodelci antibiofilmski učinek true false true MDPI AG Angleški jezik Slovenski jezik https://www.mdpi.com/journal/pharmaceutics © 2022 by the authors Članek v reviji 2025-03-24 13:41:27 2025-03-24 13:41:27 2025-03-25 03:09:42 0000-00-00 00:00:00 2022 0 0 14 str. iss. 7 (1513) Vol. 14 July 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-06-08 2022-07-18 2022-07-21 669.2/.9 116578051 10.3390/pharmaceutics14071513 1999-4923 pharmaceutics-14-01513.pdf pharmaceutics-14-01513.pdf 1 040F88E148953BBB94B8C23C1D4C5268 6f6e02b46e4a10a601ea5bbb23356d037277f3e3cea0b11e30bf1f6c7fbcd028 d7593b97-08ad-11f0-b559-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189616 https://www.mdpi.com/1999-4923/14/7/1513 0 dfd53be4-08ad-11f0-b559-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189617 Fakulteta za strojništvo 0 0 0