20.500.12556/DKUM-92246 Supercritical fluid and conventional extractions of high value-added compounds from pomegranate peels waste: production, quantification and antimicrobial activity of bioactive constituents This study is focused on different extractions (Cold Maceration (CM), Ultrasonic Extraction (UE), Soxhlet Extraction (SE) and Supercritical Fluid Extraction (SFE)) of bioactive compounds from pomegranate (Punica Granatum L.) fruit peels using methanol, ethanol, and acetone as solvents in conventional extractions and changing operating pressure (10, 15, 20, 25 MPa) in SFE, respectively. The extraction yields, total phenols (TP) and proanthocyanidins (PAC) contents, and antioxidant activity of different extracts are revealed. TP and PAC recovered by extracts ranged from 24.22 to 42.92 mg gallic acid equivalents (GAE)/g and 2.01 to 5.82 mg PAC/g, respectively. The antioxidant activity of extracts ranged from 84.70% to 94.35%. The phenolic compound identification and quantification in selective extracts was done using the LC-MS/MS method. The contents of different flavonoids and phenolic acids have been determined. SFE extract, obtained at 20 MPa, contained the highest content (11,561.84 µg/g) of analyzed total polyphenols, with predominant ellagic acid (7492.53 µg/g). For the first time, Microbial Growth Inhibition Rates (MGIRs) were determined at five different concentrations of pomegranate SFE extract against seven microorganisms. Minimal Inhibitory Concentration (MIC90) was determined as 2.7 mg/mL of SFE pomegranate peel extract in the case of five different Gram-negative and Gram-positive bacteria. antimicrobial activity antioxidants bioactive compounds extraction LC-MS/MS phenolics phytochemistry pomegranate Punica Granatum L. secondary metabolites antimikrobne lastnosti bioaktivne sestavine ekstrakcija fenoli fitokemija true false true MDPI AG Angleški jezik Slovenski jezik https://www.mdpi.com/journal/plants © 2022 by the authors Članek v reviji 2025-03-24 13:50:27 2025-03-24 13:50:28 2025-03-25 03:09:42 0000-00-00 00:00:00 2022 0 0 17 str. iss. 7 Vol. 11 March 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-03-04 2022-03-28 2022-03-30 66.02 102860803 10.3390/plants11070928 2223-7747 plants-11-00928.pdf plants-11-00928.pdf 1 4862A0508402F429AEABBEDC37AF17E0 c38c8312340878809a0fb9ae55b77b3cf4be02a99b79812ed53d178917e465b4 f7416584-08ae-11f0-b559-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189621 https://www.mdpi.com/2223-7747/11/7/928 0 fd1f5597-08ae-11f0-b559-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189622 Fakulteta za kemijo in kemijsko tehnologijo Fakulteta za strojništvo 0 0 0