20.500.12556/DKUM-92260 First insights into the antiviral activity of chitosan-based bioactive polymers towards the bacteriophage Phi6: physicochemical characterization, inactivation potential, and inhibitory mechanisms The outbreak of the worrisome coronavirus disease in 2019 has caused great concern among the global public, especially regarding the need for personal protective equipment with applied antiviral agents to reduce the spread and transmission of the virus. Thus, in our research, chitosan-based bioactive polymers as potential antiviral agents were first evaluated as colloidal macromolecular solutions by elemental analysis and charge. Three different types of low and high molecular weight chitosan (LMW Ch, HMW Ch) and a LMW Ch derivative, i.e., quaternary chitosan (quart-LMW Ch), were used. To explore their antiviral activity for subsequent use in the form of coatings, the macromolecular Chs dispersions were incubated with the model virus phi6 (surrogate for SARS-CoV-2), and the success of virus inactivation was determined. Inactivation of phi6 with some chitosan-based compounds was very successful (>6 log), and the mechanisms behind this were explored. The changes in viral morphology after incubation were observed and the changes in infrared bands position were determined. In addition, dynamic and electrophoretic light scattering studies were performed to better understand the interaction between Chs and phi6. The results allowed us to better understand the antiviral mode of action of Chs agents as a function of their physicochemical properties. bacteriophage phi6 bioactive polysaccharide biomaterials chitosans antiviral activity dynamic and electrokinetic light scattering charge biointerfaces bakteriofag phi6 bioaktivni polisaharidni biomateriali hitozan protivirusno delovanje dinamično in elektrokinetično sipanje svetlobe biomatriali true false true MDPI AG Angleški jezik Slovenski jezik https://www.mdpi.com/journal/polymers © 2022 by the authors Članek v reviji 2025-03-26 09:06:38 2025-03-26 09:06:38 2025-03-27 03:11:50 0000-00-00 00:00:00 2022 0 0 str. 1-19 iss. 16 (3357) Vol. 14 Aug. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-07-20 2022-08-11 2022-08-17 677.017:577 119204867 10.3390/polym14163357 2073-4360 polymers-14-03357.pdf polymers-14-03357.pdf 1 45DC48B140C5523CD850B5680789783E afe1f58a2e8b282a6d63fb711ad0c88e6eb8fba81b49ef1045f886e48cea1299 79c43afe-0a19-11f0-af23-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189657 https://www.mdpi.com/2073-4360/14/16/3357 0 7e545b74-0a19-11f0-af23-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189658 Fakulteta za strojništvo 0 0 0