20.500.12556/DKUM-92364 Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing Novel bioactive bacterial nanocellulose (BnC) membranes were developed for effective, non-surgical debridement and infection-prevention in burn wound healing. Membranes were modified in situ with carboxymethyl cellulose (CMC) and ex situ with the proteolytic enzyme bromelain (Br) and antimicrobial peptide nisin (N). Post-processing into stable cellulose nanocrystal dispersions (ζ = -26 mV), enables assembly of model films for demonstration of high, irreversible bromelain (95 %) and nisin (99.5 %) adsorption. The BnC-CMC and BnC-CMC-N membranes were in vitro cytocompatible for HaCaT cells and induced faster cell proliferation with cell viability exceeding 100 % after 24 h incubation. The innovative aspect of this study lies in the ex vivo evaluation using an advanced human skin explant model with induced burns, providing a realistic, physiologically relevant assessment of membrane performance. Ex vivo experiments indicated the cytocompatibility of the BnC-CMC membrane with no acute toxicity or skin irritation, while nisin presence resulted in moderate irritating effect. Notably, the BnC-CMC-Br membrane showed digestion of intercellular junctions in the epidermis, while not inducing acute toxicity and skin irritation. By leveraging this innovative ex vivo human skin model in novel BnC-based membranes testing, the study provides a crucial translational step, bridging in vitro assessments and clinical applications for burn wound treatment. bacterial nanocellulose Bromelain Nisin Carboxymethyl cellulose antimicrobial function bioactive burn wound treatment bakterijska nanoceluloza želatina nizin karboksimetil celuloza protimikrobno delovanje bioaktivnost zdravljenje opeklinskih ran true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/carbohydrate-polymer-technologies-and-applications © 2025 The Author(s) Članek v reviji 2025-04-01 15:53:00 2025-04-01 15:53:00 2026-01-22 12:16:30 0000-00-00 00:00:00 2025 0 0 20 str. [article no.] 100762 Vol. 10 June 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-03-15 677.46:620.3 231067907 10.1016/j.carpta.2025.100762 2666-8939 1-s2.0-S266689392500101X-main.pdf 1-s2.0-S266689392500101X-main.pdf 1 C9584C9A6655E876A35E2F9520D70A69 4dc69269f3fa20c2f71e773072b0f975f10e28aaa2b28c1232ae52a61aad007b 3e60f981-0f01-11f0-811a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189910 https://www.sciencedirect.com/science/article/pii/S266689392500101X 0 4494d645-0f01-11f0-811a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=189911 Fakulteta za strojništvo 0 0 0