20.500.12556/DKUM-92456 A HepG2 cell-based biosensor that uses stainless steel electrodes for hepatotoxin detection Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples. HepG2 cell line impedance biosensor adhesion hepatotoxins stainless steel biosenzorji hepatotoksini adhezija nerjaveče jeklo true false true MDPI AG Angleški jezik Slovenski jezik https://www.mdpi.com/journal/biosensors © 2022 by the authors Članek v reviji 2025-04-10 08:44:19 2025-04-10 08:44:19 2025-04-11 03:14:11 0000-00-00 00:00:00 2022 0 0 1-15 iss. 3 Vol. 12 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-02-11 2022-03-02 2022-03-04 543.2/.9 99755011 10.3390/bios12030160 2079-6374 biosensors-12-00160.pdf biosensors-12-00160.pdf 1 C2C7737734A03990D2FD88C120E817EB 309dc247f79caecae86530995c7217c150128bd824e72e6143d9f38d0f9820c4 92c8ea8b-15d7-11f0-980a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190169 https://www.mdpi.com/2079-6374/12/3/160 1 3b623c46-15d7-11f0-980a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190168 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0