20.500.12556/DKUM-92461 MFUM-BrTNBC-1, a newly established patient-derived triple-negative breast cancer cell line molecular characterisation, genetic stability, and comprehensive comparison with commercial breast cancer cell lines Triple-negative breast cancer (TNBC) is a breast cancer (BC) subtype that accounts for approximately 15–20% of all BC cases. Cancer cell lines (CLs) provide an efficient way to model the disease. We have recently isolated a patient-derived triple-negative BC CL MFUM-BrTNBC-1 and performed a detailed morphological and molecular characterisation and a comprehensive comparison with three commercial BC CLs (MCF-7, MDA-MB-231, MDA-MB-453). Light and fluorescence microscopy were used for morphological studies; immunocytochemical staining for hormone receptor, p53 and Ki67 status; RNA sequencing, qRT-PCR and STR analysis for molecular characterisation; and biomedical image analysis for comparative phenotypical analysis. The patient tissue-derived MFUMBrTNBC-1 maintained the primary triple-negative receptor status. STR analysis showed a stable and unique STR profile up to the 6th passage. MFUM-BrTNBC-1 expressed EMT transition markers and displayed changes in several cancer-related pathways (MAPK, Wnt and PI3K signalling; nucleotide excision repair; and SWI/SNF chromatin remodelling). Morphologically, MFUM-BrTNBC-1 differed from the commercial TNBC CL MDA-MB-231. The advantages of MFUM-BrTNBC-1 are its isolation from a primary tumour, rather than a metastatic site; good growth characteristics; phenotype identical to primary tissue; complete records of origin; a unique identifier; complete, unique STR profile; quantifiable morphological properties; and genetic stability up to (at least) the 6th passage. hormonal receptors MFUM-BrTNBC-1 MCF-7 MDA-MB-231 MDA-MB-453 hormonski receptorji true false true MDPI AG Angleški jezik Slovenski jezik https://www.mdpi.com/journal/cells © 2021 by the authors Članek v reviji 2025-04-10 11:21:27 2025-04-10 11:21:27 2025-04-11 03:14:11 0000-00-00 00:00:00 2022 0 0 str. [1]-23 issue 1 Vol. 11 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2021-10-14 2021-12-27 2021-12-30 618.19-006 91552259 10.3390/cells11010117 2073-4409 cells-11-00117-v2.pdf cells-11-00117-v2.pdf 1 D587CCC8173C9F1DB436AD6C1F60EF58 c42d5737cb97fc7fa75a6d14e76661f0b929302bf0a6aedbdfe381353c5bdbb2 1a672f18-15f1-11f0-980a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190183 https://www.mdpi.com/2073-4409/11/1/117 1 3065ab70-15ed-11f0-980a-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190181 Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0