20.500.12556/DKUM-92506 Pupal development and adult acclimation temperatures influence the cold and heat tolerance in Tenebrio molitor (Coleoptera: Tenebrionidae) Temperature plays a crucial role in shaping the biology of insects. Developmental temperature and acclimation temperatures influence their ability to cope with extreme thermal conditions. This study investigates the effects of developmental temperatures during the pupal stage and adult acclimation temperatures on the thermal tolerance of Tenebrio molitor Linnaeus, 1758. We investigated cold tolerance based on chill-coma recovery time and heat tolerance based on heat knockdown time. Beetles were reared at five developmental temperatures (16, 21, 25, 30 and 35 °C) and later exposed to corresponding adult acclimation temperatures. From developmental temperatures of 21 and 30 °C, the group of beetles was subjected to different temperatures to induce adult acclimation at a different temperature than the developmental temperature. In cold-tolerance tests, beetles reared at lower temperatures showed better recovery from chill coma, while beetles reared at higher temperatures showed greater resistance to heat shock. Adult beetles acclimated to lower temperatures showed better cold tolerance, while those acclimated to higher temperatures performed better in the heat tolerance test. Interestingly, the developmental temperatures during the pupal stage also contributed to resistance, particularly in the heat tolerance test. However, pupal stage temperatures had no effect on cold-shock resistance, as indicated by chill-coma recovery time. The results could provide insights into the rearing of T. molitor. adult acclimation cold tolerance developmental acclimation heat knockdown heat tolerance Tenebrio molitor thermal tolerance chill coma yellow mealworm prilagajanje odraslih toleranca na mraz razvojno prilagajanje toplotno znižanje toplotna toleranca rumeni mokar true false true Angleški jezik Slovenski jezik Znanstveno delo 2025-04-15 09:11:13 2025-04-15 09:23:11 2025-04-16 03:08:17 0000-00-00 00:00:00 2025 0 0 11 str. št. 4, št. članka 402 Letn. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-03-12 2025-04-09 2025-04-11 595.76 232765699 10.3390/insects16040402 2075-4450 RAZ_Podlesnik_Jan_2025.pdf RAZ_Podlesnik_Jan_2025.pdf 1 93C8525F5828A643675B5C7C3F2D16D0 8a040045daecd9a9e10829a081979c7ad180da5b6fc102a88afd947b4d7f23b7 680a3c9f-19ca-11f0-abed-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190297 https://doi.org/10.3390/insects16040402 1 d1da4f37-19c8-11f0-abed-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190295 Fakulteta za naravoslovje in matematiko 0 0 0