20.500.12556/DKUM-92594 Protein stacking on the APTES-functionalized pyrochlore ▫$Bi_2Ru_2O_7$▫ clusters for ultrasensitive and selective immunosensing With their unique physicochemical properties, such as metallic-like conductivity, favorable (electro)catalytic properties, electrochemical stability, and ease of functionalization, pyrochlores have found applications in various fields such as solid oxide fuel cells, batteries, thick film resistors, and temperature sensors; however, there are no reports on their application in electrochemical immunosensing. In this study, we exploited the (electro)catalytic nature and stability of the pyrochlore Bi2Ru2O7 clusters silanized with (3-aminopropyl)triethoxysilane (APTES) to demonstrate their potential for the effective stacking of functional proteins. Characterization of the clusters by XPS disclosed a dual environment of Bi, also indicating the presence of Bi2O3 alongside APTES-Bi2Ru2O7 clusters and, importantly, the predominant involvement of pyrochlore moieties in subsequent protein stacking. After stacking protein A and antibodies, the immunosensor revealed a nearly interference-free operation, high sensitivity, a detection limit of 118 fM SARS-CoV-2 spike protein, and operation in a wide examined concentration range of 10−5−10−1 μg mL−1 with an r2 of 0.98. In combination with a short incubation time of 30 min, the pyrochlore-based immunosensor provides a solid platform for future point-of-need applications. Bi2Ru2O7 pyrochlore APTES SARS-CoV-2 spike protein immunosensors electrochemical elektrokemija piroklor proteini beljakovine kataliza true false true ACS Publications Angleški jezik Slovenski jezik https://pubs.acs.org/journal/aamick?ref=breadcrumb © 2025 The Authors Članek v reviji 2025-04-23 14:46:34 2025-04-23 14:46:35 2026-01-09 03:11:03 0000-00-00 00:00:00 2025 0 0 str. 10792-10801 iss. 7 Vol. 17 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-10-16 2025-01-25 2025-02-06 543.384 228608771 10.1021/acsami.4c17869 1944-8252 protein-stacking-on-the-aptes-functionalized-pyrochlore-bi2ru2o7-clusters-for-ultrasensitive-and-selective-immunosensing.pdf protein-stacking-on-the-aptes-functionalized-pyrochlore-bi2ru2o7-clusters-for-ultrasensitive-and-selective-immunosensing.pdf 1 C9A6BAFC6F23F3F094A5B9BF80F129A7 a9a7513c9788c95defcb911e4e203bfcf3a93effdf0ac32cd3bd8fd0b584a1e1 d5d7ca4b-ec82-11f0-a4e9-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201832 https://pubs.acs.org/doi/10.1021/acsami.4c17869 1 feb11e15-2040-11f0-adc7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190483 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0