20.500.12556/DKUM-92747 Optimisation of the green process of industrial hemp - preparation and its extract characterisation Natural medicines and products are becoming increasingly important in the pharmaceutical and food industries. The most important step in obtaining a natural remedy is the processing of the natural material. This study offers the separation of the industrial hemp plant into fractions by mechanical treatment, which has a significant impact on the selectivity of the obtained fractions. This study also offers a solution to reduce waste by fractionating industrial hemp, focusing on the fraction with the highest cannabinoid content (49.5% of CBD). The study confirmed the anticancer potential of the extract, which prevents further division of WM-266-4 melanoma cells at a concentration of 10−3 mg/mL. However, application of the extract (c = 10−3 mg/mL) to normal human epidermal melanocytes proved to be insignificant, as the metabolic activity of the cells was the same as in the control cell group. supercritical fluid extraction ultrasonic extraction melanoma cells extraction WM-266-4 superkritična ekstrakcija tekočin ultrasonična ekstrakcija ekstrakcija true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/plants © 2022 by the authors Članek v reviji 2025-05-09 14:06:16 2025-05-09 14:06:16 2025-05-10 03:10:00 0000-00-00 00:00:00 2022 0 0 14 str. iss. 13 Vol. 11 June 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-06-15 2022-06-28 2022-06-30 66.02 113614339 10.3390/plants11131749 2223-7747 plants-11-01749-v3.pdf plants-11-01749-v3.pdf 1 2A266CB55E560962A56A3E6E178770A2 8400098c98179150a351a21947997cc0c46e95d1f1b474745f5e9e0254498d92 42b3db94-2cce-11f0-acfb-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190842 https://www.mdpi.com/2223-7747/11/13/1749 0 487317cb-2cce-11f0-acfb-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190843 Fakulteta za kemijo in kemijsko tehnologijo Fakulteta za strojništvo Medicinska fakulteta 0 0 0