20.500.12556/DKUM-92749 Synergistic effect of supercritical and ultrasound-assisted ginger (Zingiber officinale Roscoe) extracts Proper processing of natural material is crucial to obtain an extract with high content of biologically active components. Dried, grinded ginger roots were extracted by ultrasonic method and supercritical extraction with CO2 . The aim of the study was to determine if a mixture of the two types of extracts attained by different methods and solvents exhibits better bioavailability than each extract itself. Therefore, both extracts were analytically evaluated and then mixed in a ratio of 1:1. The supercritical extract (SCG extract) and the mixed extract (mixG extract) had high antioxidant activity (78% and 73%) and total phenols (827 mg/g ext. and 1455 mg/g ext.), which is also consistent with the levels of gingerol (303 mg/g ext. and 271 g/g ext.) and shogaol (111 mg/g ext. and 100 g/g ext.) in the extracts. In comparison to both pure extracts higher levels of total phenols were found in the extract mixG. This could be the reason for the significant inhibition of melanoma cells and antimicrobial potential (against Staphylococcus aureus, Escherichia coli, and Candida albicans). The combination of the extracts resulted in a significant increase in the inhibition of selected microbial and melanoma cells WM-266-4 compared to the control. Cell viability decreased below 60% when mixG extract was applied. Antimicrobial activity has been confirmed. ginger supercritical fluid extraction ultrasound-assisted extraction antimicrobial activity ingver ekstrakcija superkritičnih fluidov ultrasonična ekstrakcija antimikrobne aktivnosti true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/plants © 2022 by the authors Članek v reviji 2025-05-09 14:15:17 2025-05-09 14:15:17 2025-05-10 03:10:00 0000-00-00 00:00:00 2022 0 0 14 str. iss. 21 Vol. 11 27 Oct. 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-10-05 2022-10-24 2022-10-27 66.02 128508675 10.3390/plants11212872 2223-7747 plants-11-02872-v2.pdf plants-11-02872-v2.pdf 1 F7C38393EFB0BEE09CD029CD87F9BFD4 780ccee1401be5380b4f5e624a7716d2c3156cf0de2013b742d883736a3841da a9977c1f-2ccf-11f0-acfb-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190844 https://www.mdpi.com/2223-7747/11/21/2872 0 af96495e-2ccf-11f0-acfb-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=190845 Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0