20.500.12556/DKUM-92818 The effect of preconditioning strategies on the adsorption of model proteins onto screen-printed carbon electrodes The preconditioning and modification of the supporting electrode surface is an essential step in every biosensor architecture. In particular, when using screen-printed carbon electrodes (SPEs) as inexpensive and convenient disposable sensor substrates, their somewhat lower electrochemical (surface) reproducibility might represent a complex hurdle. Herein, we investigated the effect of selected preconditioning strategies, such as cyclic voltammetric pretreatment, in H2SO4 and H2O2 and plasma pretreatment with a positive and negative glow discharge, which all improved the electrochemical stability of the unmodified SPEs. Furthermore, we studied the influence of preconditioning strategies on the adsorption kinetics of the two most commonly used building blocks for biosensor preparation, i.e., bovine serum albumin (BSA) and protein A. We observed an advantageous effect of all the examined preconditioning strategies for the modification of SPEs with protein A, being the most effective the negative glow discharge. On the other hand, BSA exhibited a more complex adsorption behavior, with the negative glow discharge as the only generally beneficial preconditioning strategy providing the highest electrochemical stability. Protein A revealed a more substantial impact on the electrochemical signal attenuation than BSA considering their same concentrations in the modification solutions. For both BSA and protein A, we showed that the concentrations of 5 and 10 µg mL−1 already suffice for an electrochemically satisfactorily stable electrode surface after 60 min of incubation time, except for BSA at the positive-plasma-treated electrode. screen-printed carbon electrode SPE electrochemical biosensor adsorption bovine serum albumin protein A glow discharge karbonske elektrode proteini adsorpcija true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/sensors © 2022 by the authors Članek v reviji 2025-05-15 08:57:46 2025-05-15 08:57:46 2025-05-16 03:10:53 0000-00-00 00:00:00 2022 0 0 14 str. iss. 11 Vol. 22 2022 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2022-05-03 2022-05-27 2022-05-31 544.5/.6 110592003 10.3390/s22114186 1424-8220 sensors-22-04186-v2.pdf sensors-22-04186-v2.pdf 1 0A90D278BD1CC626EECF7E5941D7CECE 108eff93838d50329d35528a75d4a794437af3719d4dd88726cc08b0dc07a53a d01f20e5-315a-11f0-832c-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=191029 https://www.mdpi.com/1424-8220/22/11/4186 0 d9aafe9a-315a-11f0-832c-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=191030 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0