20.500.12556/DKUM-92936 Chlorpyrifos detection based on 9-fluorenone oxime Chlorpyrifos is one of the most toxic organophosphate pesticides, prompting its ban in Europe in 2020. Consequently, developing a detection method that is both selective and sensitive is essential for protecting human health and the environment. In this study, we report for the first time a fluorescent probe based on an oxime for the direct detection of chlorpyrifos. 9-fluorenone oxime, upon deprotonation with a phosphazene base, undergoes a nucleophilic attack on chlorpyrifos, resulting in a significant alteration of its fluorescence properties. Following careful optimization, the method demonstrated excellent linearity (R2 = 0.98) over a concentration range of 350 to 6980 μg/L, with a limit of detection of 15.5 μg/L. Furthermore, the probe was successfully applied to chlorpyrifos detection in water samples, yielding satisfactory results. This approach effectively overcomes the stability limitations of enzyme-based fluorescent sensors, offering a robust and innovative solution for the detection of organophosphate pesticides. organophosphate pesticide chlorpyrifos fluorenone oxime fluorescence phosphazene organofosfatni pesticidi klorpirifos fluorenon oksim fluorescenca fosfazen true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/2227-9040/13/5/170 Članek v reviji 2025-05-27 16:31:04 2025-05-27 16:31:04 2025-05-28 03:09:17 0000-00-00 00:00:00 2025 0 0 12 str. iss. 5, [article no.] 170 Vol. 13 May 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-05-02 2025-05-02 2025-05-06 544 237411075 10.3390/chemosensors13050170 2227-9040 chemosensors-13-00170-v2.pdf chemosensors-13-00170-v2.pdf 1 E8D3577EA27ABDEC84DE5EC4E607FF3A 1be1fcc4597670a9fdc1d041961d30dbf699686c3c397501187971dbdddcafc7 7b2d9e4d-3b07-11f0-be37-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=191316 https://www.mdpi.com/2227-9040/13/5/170 0 81322424-3b07-11f0-be37-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=191317 Fakulteta za strojništvo 0 0 0