20.500.12556/DKUM-93001 Surface and subsurface mass spectrometric analysis of dexamethasone in solid pharmaceutical dosage forms This study presents an in-depth mass spectrometric investigation of dexamethasone (DEX) distribution within pharmaceutical tablets using time-of-flight secondary ion mass spectrometry (ToF-SIMS) combined with gas cluster ion beam (GCIB) sputtering. Fragmentation mechanism of DEX was identified, which enabled the determination of three-dimensional chemical imaging of the active ingredient in both surface and subsurface regions. The data reveal that a 4-mg DEX formulation exhibits a continuous and extended distribution of the drug into the tablet matrix, while a 0.5-mg formulation shows DEX localized in distinct, isolated domains. Topographical features and the overall composition of the surface were confirmed by complementary analyses employing atomic force microscopy (AFM) and x-ray photoelectron spectroscopy (XPS). These results demonstrate how molecule distribution patterns can be linked to formulation heterogeneity using advanced mass spectrometric techniques, opening new possibilities for pharmaceutical manufacturing quality control and optimization. mass spectrometry dexamethasone pharmaceutical tablets DEX ToF-SIMS masna spektrometrija deksametazon zdravila true true false Wiley Angleški jezik Slovenski jezik https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jms.5147 © 2025 The Author(s) Članek v reviji 2025-05-30 11:41:45 2025-05-30 11:41:45 2025-05-31 03:09:14 0000-00-00 00:00:00 2025 0 0 11 str. First published: 18 May 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-05-08 2025-05-13 2025-05-18 66 236328963 10.1002/jms.5147 1096-9888 Journal_of_Mass_Spectrometry_-_2025_-_Finsgar_-_Surface_and_Subsurface_Mass_Spectrometric_Analysis_of_Dexamethasone_in.pdf Journal_of_Mass_Spectrometry_-_2025_-_Finsgar_-_Surface_and_Subsurface_Mass_Spectrometric_Analysis_of_Dexamethasone_in.pdf 1 1006D2650BF3F3FF64B6E7EBF84D3798 aba94614d0a64dad8965f58c5a12ee824521fc35195bd4e96c64cf5efba675b9 909be2a7-3d3a-11f0-adaa-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=191534 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0