20.500.12556/DKUM-93091 Termodinamske lastnosti in formulacije cbd za sproščanje v gastrointestinalnem sistemu magistrsko delo Thermodynamic properties and formulations of cbd for release in the gastrointestinal system CBD je nepsihoaktiven fitokanabinoid, pridobljen iz konoplje, ki izkazuje številne farmakološke lastnosti, kot so protivnetne, antimikrobne, antioksidativne in citoprotektivne. Zaradi nizke topnosti v vodi ima omejeno biološko razpoložljivost. Namen magistrske naloge je bil pripraviti formulacije, ki omogočijo boljšo biološko uporabnost CBD-ja. V okviru magistrskega dela smo najprej ovrednotili topnost CBD-ja v superkritičnem CO2 pri temperaturah 40 °C, 60 °C in 80 °C ter tlakih med 100 bar in 600 bar. Na podlagi pridobljenih rezultatov o topnosti smo izvedli PGSSTM postopek z različnimi razmerji CBD/PEG 4000. Poleg tega smo pripravili tudi organogele, hidrogele in suspenziji, ki temeljita na sestavah konvencionalnih zdravil Epidiolex in Sativex. Preliminarna študija sproščanja v simuliranem črevesnem sistemu je pokazala, da je PGSSTM formulacija omogočila najbolj učinkovito sprostitev CBD, kar je vidno iz izrazitih spektralnih vrhov. Sproščanje je zaznano tudi pri suspenzijah, medtem ko pri organogelih in hidrogelih ni bilo zaznane sprostitve aktivne komponente. Rezultati antimikrobne aktivnosti so pokazali, da PGSSTM formulacija najbolj učinkovito zavira rast Gram pozitivne bakterije Straphylococcus aureus (sev MRSA) z minimalno inhibitorno koncentracijo (MIC) 1,95 μg CBD/mL gojišča. Antimikrobna aktivnost je bila opažena tudi pri eni vrsti suspenzije in dveh organogelih, vendar z dvakrat oziroma štirikrat višjimi vrednostmi MIC. Nobena formulacija ni bila učinkovita proti kvasovki Candida albicans ali Gram negativni bakteriji Escherichia coli. Kot najuspešnejša formulacija se je izkazala PGSSTM, ki predstavlja inovativno in doslej neraziskano možnost izboljšanja topnosti in posledično biološke razpoložljivosti CBD. CBD is a non-psychoactive phytocannabinoid derived from hemp, exhibiting numerous pharmacological properties, including anti-inflammatory, antimicrobial, antioxidant, and cytoprotective effects. Due to its low water solubility, it has limited bioavailability. The aim of this master's thesis was to develop formulations that improve the bioavailability of CBD. As part of experimental work, we first evaluated the solubility of CBD in supercritical CO2 at temperatures of 40 °C, 60 °C, and 80 °C, and pressures ranging from 100 bar to 600 bar. Based on the obtained solubility data, we performed the PGSSTM method using different CBD/PEG 4000 ratios. In addition, we prepared organogels, hydrogels, and suspensions based on the compositions of conventional drugs Epidiolex and Sativex. A preliminary release study in a simulated intestinal system showed that the PGSSTM formulation enabled the most effective CBD release, as evidenced by pronounced spectral peaks. Release was also detected in the suspensions, while organogels and hydrogels showed no detectable release of the active component. Antimicrobial activity results indicated that the PGSSTM formulation most effectively inhibited the growth of the Gram-positive bacterium Staphylococcus aureus (MRSA strain), with a minimum inhibitory concentration (MIC) of 1.95 μg CBD/mL of culture medium. Antimicrobial activity was also observed in one type of suspension and two organogels, albeit with MIC values two to four times higher than that of the PGSSTM formulation. None of the formulations showed efficacy against the yeast Candida albicans or the Gram-negative bacterium Escherichia coli. The PGSSTM formulation proved to be the most successful, representing an innovative and previously unexplored approach to improving the solubility and consequently the bioavailability of CBD. CBD minimalna inhibitorna koncentracija formulacije sproščanje topnost CBD minimum inhibitory concentration formulations release solubility true true false [A. Rahmanović] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-06-05 10:42:34 2025-06-24 13:47:32 2025-07-24 03:09:25 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XII, 43 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 [544.351.3:582.630]:615.281(043.2) 243499011 MAG_Rahmanovic_Armina_2025.pdf MAG_Rahmanovic_Armina_2025.pdf 1 F5353CD3F8F592ABADA9DFFC2561BAF6 e84bf9e2dddca895a799284762a9c1ceea545209b1d789a7eaa6b4dac83588c5 67d2590f-5056-11f0-a7a4-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=192638 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0