20.500.12556/DKUM-93549 Ekstrakcija β-karotena in fukoksantina iz morskih alg diplomsko delo univerzitetnega študijskega programa I. stopnje Extraction of β-carotene and fucoxanthin from sea algae Diplomsko delo se osredotoča na optimizacijo superkritične ekstrakcije β-karotena in fukoksantina iz alg, s predhodno obdelavo teh z ionsko tekočino holin lizinat. Tako β-karoten kot fukoksantin, sta pomembna karotenoida z velikim potencialom v farmacevtski in prehrambni industriji. Parametra, na podlagi katerih smo pripravili eksperimentalni načrt, sta bila tlak in temperatura, kjer smo z njuno variacijo optimizirali vsebnost fukoksantina in β-karotena v ekstraktih. V primerjavi z drugimi metodami, je bila vsebnost v ekstraktih večja z uporabo superkritične ekstrakcije. Optimalni pogoji te metode so bili 350 bar in 50 °C, kjer je bila vsebnost fukoksantina 31,34 mg Fx/g ekstrakta, vsebnost β-karotena pa <1 mg Bc/g ekstrakta. Tudi v vseh ostalih ekstraktih nismo zaznali β-karotena nad mejo detekcije. Dobljene rezultate smo nato primerjali z drugimi študijami, s katerimi smo ugotovili, da pred obdelava z ionsko tekočino holin lizinat minimalno pripomore k učinku na količino ekstrahiranega fukoksantina ali β-karotena. Ti so nam prav tako pokazali, da je superkritična ekstrakcija s pred obdelavo z ionsko tekočino, učinkovitejša od vroče maceracije in Soxhletove ekstrakcije. The thesis focuses on the optimisation of supercritical extraction of β-carotene and fucoxanthin from algae by pretreating them with the ionic liquid choline lysinate. Both β-carotene and fucoxanthin are important carotenoids with great potential in the pharmaceutical and food industries. The parameters on the basis of which the experimental design was prepared were pressure and temperature, and their variation was used to optimise the fucoxanthin and β-carotene content in the extracts. Compared to other methods, the content in the extracts was higher using supercritical extraction. The optimum conditions for this method were 350 bar and 50 °C, where the fucoxanthin content was 31,34 mg Fx/g extract and the β-carotene content was <1 mg Bc/g extract. In all other extracts, β-carotene was not detected above the detection limit. The results obtained were then compared with other studies, which concluded that pre-treatment with the ionic liquid choline lysinate contributes minimally to the effect on the amount of fucoxanthin or β-carotene extracted. They also showed us that supercritical extraction with ionic liquid pre-treatment is more efficient than hot maceration and Soxhlet extraction. fukoksantin β-karoten superkritična ekstrakcija pigmenti izolacija fucoxanthin β-carotene supercritical extraction pigments isolation true true false [G. Korda] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-07-02 09:25:22 2025-07-10 12:13:00 2025-07-25 03:12:04 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IX, 24 f.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.061:547.979.8(043.2) 243665667 UN_Korda_Gal_2025.pdf UN_Korda_Gal_2025.pdf 1 015BB1C812A5299DC583AD5100F40D56 dc4d84e6a56fb0b505f2a54a31d24b0986a7669f079033c376ad011b7f677f56 633617b6-5d73-11f0-b0f7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=193459 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0