20.500.12556/DKUM-93711 Substrate-dependent variability in viability and angiogenicmarker expression among three endothelial cell subtypes insights for artificial tissue vascularization issue engineering faces the challenge of achieving effective vascularization within tissue constructs for sustained viability andoptimal function. The success of tissue-engineered constructs depends on selecting an optimal angiogenesis-stimulating ECMsubstitute material. This study compares four substrates made from three different biomacromolecules—fibrin, fibronectin,non-crosslinked, and crosslinked gelatin, and their effect on endothelial cells. Acknowledging the diverse range of endothelialcells that play a role in (micro)vascularization, human endothelial primary cells, human umbilical vein endothelial cells, andhuman microvascular endothelial cells are subjected to these materials for evaluation. Biocompatibility is assessed by measuringcell viability (Live/Dead assay), metabolic activity (alamarBlue assay), morphology (actin staining), phenotype expression(immunocytochemistry), and the production of von Willebrand factor, which promotes angiogenesis by promoting cell adhesionand migration. The results show that the use of biomaterials as culturing substrates significantly impacts the viability andmorphology of the cells. While the expression of angiogenic markers is shown to rely more on the cell lineage, the use of differentsubstrates has an impact on the expression timeline. Thus, combining cells and biomaterials in a favorable manner can be usedas a powerful tool for controlled vascularization in vitro, which requires the systematic assembly of different stimuli. biomacromolecules endothelial cell types materials microvascularization vascularization biomakromolekule endotelijske celice materiali mikrovaskularizacija vaskularizacija true false true Wiley-VCH Angleški jezik Slovenski jezik https://onlinelibrary.wiley.com/journal/16165195 © 2025 The Author(s). Članek v reviji 2025-07-16 03:09:39 2025-07-22 07:40:07 2025-07-23 03:11:06 0000-00-00 00:00:00 2025 0 0 str. 1-18 iss. 7 Vol. 25 Jul. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-05-29 2025-06-18 2025-07-10 61 242531843 10.1002/mabi.202500333 1616-5187 Macromolecular_Bioscience_-_2025_-_Vajda_-_Substrate‐Dependent_Variability_in_Viability_and_Angiogenic_Marker_Expression.pdf Macromolecular_Bioscience_-_2025_-_Vajda_-_Substrate‐Dependent_Variability_in_Viability_and_Angiogenic_Marker_Expression.pdf 1 79904CAB94EE0ABEB5FEBD9B7C1D7D06 e96f22c4289cb7680c4080b59d38a853741a6162339776b8c062e58426a2c45f f58f8c8b-66be-11f0-b4a7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=193886 https://onlinelibrary.wiley.com/doi/10.1002/mabi.202500333 0 fe1e73c1-66be-11f0-b4a7-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=193887 Medicinska fakulteta Fakulteta za kemijo in kemijsko tehnologijo 0 0 0