20.500.12556/DKUM-93765 Siderophore production, diversity of siderophore receptors and associations with virulence-associated genes, phylogroups and bacteriocin production in Escherichia coli strains isolated from humans, animals and organic fertilizers Iron uptake plays an important role in the persistence of Escherichia coli in the host and for its survival in the environment, and it is known that E. coli has a variety of siderophore systems for iron uptake. We investigated the ability to produce siderophores, the genetic diversity of the siderophores and their correlation with virulence-associated genes (VAGs), phylogroups and bacteriocin production in E. coli strains isolated from different sources: uropathogenic E. coli (UPEC) from urine of patients with urinary tract infections, avian pathogenic E. coli (APEC) from organs of birds with signs of colibacillosis, fecal E. coli (FEC) from feces of healthy cattle and E. coli from organic fertilizers based on poultry and cattle manure (OFEC). A high variability in siderophore production was found among the UPEC strains studied, while the OFEC strains showed the highest siderophore production among all groups. Genes for aerobactin and yersiniabactin receptors were most frequently found in the UPEC strain, followed by the APEC, FEC and OFEC strains. The greatest diversity of siderophore receptors was found in the APEC strain. We also found that iutA-positive E. coli isolated from animals contained more VAGs than iutA-negative strains. The profiles of the siderophore genes of APEC and OFEC from poultry manure were very similar, indicating that APEC can be transmitted via organic fertilizers, suggesting that poultry manure is an environmental risk. The data obtained complement the information on the prevalence of siderophore producers and contribute to our knowledge on the biodiversity of E. coli pathotypes. siderophore Escherichia coli virulence-associated genes phylogenetic group bacteriocin microcin geni povezani z virulenco filogenetska skupina bakteriocin mikrocin true true true MDPI Angleški jezik Slovenski jezik Znanstveno delo 2025-07-18 10:50:18 2025-07-18 10:50:18 2025-07-23 13:11:51 0000-00-00 00:00:00 2025 0 0 14 str. št. 2, št. članka 50 Letn. 16 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-12-30 2025-02-11 2025-02-17 579.2 227345923 2036-7481 RAZ_Kuznetsova_Marina_V._2025.pdf RAZ_Kuznetsova_Marina_V._2025.pdf 1 8AE0EEC05A138BE2547C91999B15CE2C 4f2558303c33e3c4c2a0a909205ecedc87534c42a2939ac785b6a7014a4ebce7 b0ee09e4-63b4-11f0-9d87-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=193775 https://doi.org/10.3390/microbiolres16020050 1 3bd923ef-63b4-11f0-9d87-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=193774 Fakulteta za naravoslovje in matematiko 0 0 0