20.500.12556/DKUM-93998 Razvoj in vitro modela kože Development of an in Vitro Skin Model V raziskavi smo razvili biočrnilo za 3D tiskanje z vključitvijo človeških kožnih celic, sestavljeno iz alginata, metilceluloze in nanofibrilarne celuloze v celičnem mediju. 3D biotiskalnik omogoča ustvarjanje večslojnih ogrodij, ki simulirajo naravno celično mikrookolje kože. Zaradi potrebe po ponovljivosti smo biočrnilo hranili v zamrzovalniku in ga kasneje odtajali. Reološke lastnosti materiala po odtalitvi bistveno vplivajo na njegovo viskoznost, kar vpliva na možnost 3D tiskanja in stabilnost natisnjenih ogrodij. Ugotovili smo, da temperatura zamrzovanja pomembno vpliva na te lastnosti, kar je ključno za nadaljnji razvoj in vitro modela kože, ki je obetaven za predklinične in klinične raziskave. In this study, we developed bioink for 3D printing incorporating human skin cells, composed of alginate, methylcellulose, and nanofibrillar cellulose in a cell medium. The 3D bioprinter allows the creation of multilayer scaffolds that simulate the natural cellular microenvironment of the skin. Due to the need for reproducibility, we stored the bioink in a freezer and thawed it later. The rheological properties of the material after thawing significantly affect its viscosity, impacting the feasibility of 3D printing and the stability of the printed scaffolds. We found that the freezing temperature notably influences these properties, which is crucial for the further development of an in vitro skin model, promising for preclinical and clinical research. in vitro model kože reologija 3D tiskanje predklinična medicina kožne bolezni in vitro skin model rheology 3D printing preclinical medicine skin diseases true false true Univerza v Mariboru, Univerzitetna založba Slovenski jezik Angleški jezik Znanstveno delo 2025-07-29 03:11:39 2026-02-25 10:13:04 2026-03-27 15:13:59 0000-00-00 00:00:00 2024 0 Maribor 0 19 št.1 Letn.14 maj 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-03-07 2024-04-10 2024-05-31 616.5 197650947 10.18690/analipazu.14.1.29-47.2024 2820-364X Development_of_an_in_vitro_skin-Skrinjar-2024.pdf Development_of_an_in_vitro_skin-Skrinjar-2024.pdf 1 4E6017305BA5927FB0D4D54989C5B910 8ce7664a85385b435c11e34be4ddd946fb9e5a006fc5ee45e921a5a4d0a2b9b1 731bd4bb-122a-11f1-a540-00155d580e1a https://dk.um.si/Dokument.php?lang=slv&id=203824 https://journals.um.si/index.php/anali-pazu/article/view/4436 0 fb5f038e-6c18-11f0-aa9e-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=194365 https://www.dlib.si/details/URN:NBN:SI:DOC-XKLUQGFK 0 63b77d24-122a-11f1-a540-00155d580e1a https://dk.um.si/Dokument.php?lang=slv&id=203823 Medicinska fakulteta Univerzitetna založba Univerze v Mariboru Anali PAZU 0 0 0