20.500.12556/DKUM-94075 Vpliv dodane kokosove vode na razraščanje malinjakov v tkivni kulturi diplomsko delo The Effect of Added Coconut Water on the Proliferation of Raspberries in Tissue Culture Poskus je potekal na Fakulteti za kmetijstvo in biosistemske vede Univerze v Mariboru v obdobju od maja do junija 2025. V raziskavo smo vključili tri različna gojišča: G₁ (kontrolno gojišče brez dodatka rastnih regulatorjev), G₂ (gojišče z dodatkom 1 mg/l BAP) ter G₃ (gojišče z dodatkom 10 % kokosove vode). Skupno smo testirali 120 izsečkov malinjaka, po 40 na posamezno gojišče. Osrednji cilj raziskave je bil preučiti vpliv različnih sestav gojišč na rast in razraščanje poganjkov v in vitro pogojih, s poudarkom na primerjavi učinkovitosti rastnega regulatorja citokinina BAP in naravne sestavine kokosove vode v primerjavi s kontrolnim gojiščem brez dodanih rastnih regulatorjev. Rezultati so pokazali, da so se na kontrolnem gojišču G₁ razvili večji poganjki z dobro razvitim koreninskim sistemom, bakterijska kontaminacija pa je bila omejena predvsem na območje korenin. Gojišče G₂ je spodbudilo največje število novo tvorjenih poganjkov, bakterijska kontaminacija je bila nekoliko bolj razširjena. Gojišče G₃ je omogočilo zmeren razvoj listov, hkrati pa je bilo opaziti največjo bakterijsko kontaminacijo, kar je pripisano bogati hranilni sestavi kokosove vode, ki spodbuja rast mikroorganizmov. Ugotovljeno je bilo, da čeprav kokosova voda podpira rast rastlinskih tkiv, njena uporaba povečuje tveganje za bakterijsko kontaminacijo, kar lahko posledično oteži rast in razmnoževanje v in vitro pogojih. V okviru našega poskusa se je gojišče z dodatkom BAP izkazalo kot najučinkovitejše pri spodbujanju razmnoževanja, medtem ko uporaba kokosove vode zahteva previdno načrtovanje in optimizacijo, saj lahko zaradi svoje hranilne sestave pospeši rast nezaželenih mikroorganizmov. The experiment was conducted at the Faculty of Agriculture and Life Sciences from, University of Maribor, from May to June 2025. The study included three different culture media: G₁ (control medium without added growth regulators), G₂ (medium supplemented with 1 mg/l BAP), and G₃ (medium supplemented with 10 % coconut water). A total of 120 raspberry explants were tested, with 40 explants per culture medium. The main objective of the research was to examine the effects of different media compositions on shoot growth and proliferation under in vitro conditions, with a focus on comparing the effectiveness of the cytokinin growth regulator BAP and the natural component coconut water, with the control medium without added growth regulators. The results indicated that the control medium G₁ produced larger shoots with a well-developed root system, while bacterial contamination was mainly limited to the root area. The medium G₂ promoted the highest number of newly formed shoots, although bacterial contamination was slightly more widespread. Medium G₃ enabled moderate leaf development, but exhibited the highest level of bacterial contamination, which is attributed to the rich nutrient composition of coconut water that promotes microbial growth. It was found, that although coconut water supports the growth of plant tissues, its use increases the risk of bacterial contamination, which may consequently hinder growth and propagation under in vitro conditions. Within the scope of our experiment, the medium supplemented with BAP proved to be the most effective in promoting propagation, while the use of coconut water requires careful planning and optimization due to its nutrient composition, which can accelerate the growth of undesirable microorganisms. malinjak kokosova voda in vitro tkivne kulture Rubus idaeus raspberry coconut water in vitro tissue cultures Rubus idaeus true true false Z. Orter Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-07-30 13:53:57 2025-09-04 13:24:19 2025-09-05 03:27:06 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (VIII, 46, [15] str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.711:631.533(043.2)=163.6 247688451 VS_Orter_Zala_2025.pdf VS_Orter_Zala_2025.pdf 1 BE2BD70DBCE63915CF9739F5FB7FA4D1 f9825197b40f2909a66e299c2f1b8fc26f1a0d167a57a1c86a5027c31e8d494a 9984c8ca-75cc-11f0-a01d-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=194999 Fakulteta za kmetijstvo in biosistemske vede 0 0 0