20.500.12556/DKUM-94098 Analiza in primerjava morfološke ter genetske raznolikosti vinogradniških breskev (Prunus persica (L.) Batsch) z uporabo fenotipskih in mikrosatelitskih markerjev magistrsko delo Analysis and Comparison of Morphological and Genetic Vineyard Peach (Prunus persica (L.) Batsch) Diversity Using Phenotypic and Microsatellite Markers Vinogradniške breskve (Prunus persica (L.) Batsch) predstavljajo bogat vir lokalno prilagojenega genskega materiala z bogato genetsko variabilnostjo. Ohranjanje te raznolikosti je pomembno za žlahtniteljske programe, izboljšanje in prilagajanje lastnosti kultivarjev in podlag. Za učinkovito upravljanje in rabo teh virov je bistvenega pomena natančna morfološka in genetska karakterizacija akcesij, ki jih hranimo v genskih bankah. Namen magistrske naloge je bil analizirati morfološko in genetsko raznolikost 24 akcesij vinogradniških breskev iz genske banke koščičarjev. Morfološko vrednotenje je potekalo na osnovi 25 fenotipskih deskriptorjev, genetska analiza pa z uporabo devetih mikrosatelitskih (SSR) markerjev. Na osnovi morfoloških podatkov smo s hierarhično metodo AGNES (angl. Agglomerative Nesting) razvrstili akcesije v štiri skupine, medtem ko smo z metodo NJ (angl. Neighbor-Joining tree) na osnovi genotipskih podatkov oblikovali tri skupine. Analiza SSR-markerjev je pokazala, da so bili štirje lokusi (EMPaS06, EMPaS14, BPPCT014, BPPCT034) pri vseh akcesijah monomorfni, kar kaže na omejeno diskriminatorno moč teh markerjev pri proučevanem materialu. Najbolj informativen lokus je bil EMPaS12, s štirimi aleli in vrednostjo PIC 0,474. Povprečna opažena heterozigotnost (Ho) je znašala 0,093, pričakovana heterozigotnost (He) pa 0,166, kar kaže na nizko stopnjo genetske variabilnosti znotraj analiziranega nabora. Primerjava dendrogramov, izdelanih na osnovi morfoloških in genetskih podatkov, je pokazala delno skladnost, z nekaj izjemami. Vineyard peaches (Prunus persica (L.) Batsch) are a rich source of locally adapted genetic material with a rich genetic variability. Conservation of this diversity is important for breeding programs, improving and adapting the characteristics of cultivars and rootstocks. For the effective management and use of these resources, accurate morphological and genetic characterization of accessions stored in gene banks is essential. The purpose of the master's thesis was to analyze the morphological and genetic diversity of 24 vineyard peach accessions from the stone fruit gene bank. Morphological evaluation was based on 25 phenotype descriptors, and genetic analysis was performed using nine microsatellite (SSR) markers. Based on morphological data, we classified the accessions into four groups using the hierarchical AGNES (Agglomerative Nesting) method, while three groups were formed using the NJ (Neighbor-Joining tree) method based on genotypic data. Analysis of SSR markers showed that four loci (EMPaS06, EMPaS14, BPPCT014, BPPCT034) were monomorphic in all accessions, indicating the limited discriminatory power of these markers in the studied material. The most informative locus was EMPaS12, with four alleles and a PIC value of 0.474. The average observed heterozygosity (Ho) was 0.093, and the expected heterozygosity (He) was 0.166, indicating a low level of genetic variability within the analyzed set. Comparison of dendrograms constructed based on morphological and genetic data showed partial agreement, with a few exceptions. genetska raznolikost vinogradniška breskev fenotipski markerji mikrosatelitski markerji genetic diversity vineyard peach phenotypic markers microsatellite markers true true false G. Ernecl Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-08-01 12:52:36 2025-09-08 09:01:59 2025-09-09 03:36:23 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (XIV, 98, [6] str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.25:575.2:577.21(043)=163.6 247977731 MAG_Ernecl_Gaja_2025.pdf MAG_Ernecl_Gaja_2025.pdf 1 2C7B517E31A18C4E2BF07A33A2275EB0 4228aa36c626760e833d34b494507c47c7e1dca11198da7352d9bc8633e88282 8c3e8d3a-7e91-11f0-9610-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=195862 Fakulteta za kmetijstvo in biosistemske vede 0 0 0