20.500.12556/DKUM-94867 Kemijske lastnosti strupa Kranjske sivke in njegova uporaba v kozmetičnih produktih diplomsko delo visokošolskega strokovnega študijskega programa I. stopnje Chemical properties of carniolan honeybee venom and its usage in cosmetical products Strup Kranjske sivke je manj raziskana surovina, ki postaja vedno popularna zaradi svojih lastnosti, katere so pomembne tako v kozmetiki kot v zdravstvu. Zato smo se v okviru te diplomske naloge odločili raziskati nekatere osnovnejše kemijske lastnosti čebeljega strupa, kot so vsebnost proteinov, antioksidativna aktivnost in protivnetna aktivnost. Pri tem smo primerjali pet vzorcev, ki so se razlikovali po času nabiranja, glede na obdobja paše čebel, in po mestu nabiranja strupa. Hkrati pa smo preverili obstojnost primera emulzije in hidrogela, ki vsebujeta raziskovano učinkovino. Rezultati uporabljene Bradfordove metode so pokazali visoko vsebnost proteinov v vzorcih, ki je znašala približno 90 %.To je bilo pričakovano glede na sestavo, ki jo podajajo članki na temo strupa medonosnih čebel. Podobno temu so rezultati antioksidativne analize z DPPH radikalom in protivnetne aktivnosti, dokazane z metodo, ki uporablja jajčni albumin, pokazali pričakovane vrednosti glede na opise drugih avtorjev. Pri tem je test antioksidativne aktivnosti pokazal 50 % inhibicijo pri koncentraciji okoli 30 mg/mL, z odstopanjem dveh vzorcev, zaradi prisotnih nečistoč. Test protivnetne aktivnosti pa je pokazal od 30 do 45 % inhibicijo. The venom of the Carniolan honey bee is a less researched raw material that is becoming increasingly popular due to its properties, which are important both in cosmetics and in healthcare. Therefore, within the scope of this thesis, we decided to investigate some of the basic chemical properties of bee venom, such as protein content, antioxidant activity, and anti-inflammatory activity. In doing so, we compared five samples that differed according to the time of collection, based on the bees’ foraging periods, as well as the location of venom collection. At the same time, we examined the stability of an emulsion and a hydrogel containing the studied active substance. The results of the Bradford method showed a high protein content in the samples, amounting to approximately 90%. This was expected given the composition reported in articles on honey bee venom. Similarly, the results of the antioxidant analysis using the DPPH radical, as well as the anti-inflammatory activity proven by the method based on egg albumin, showed values consistent with those reported by other authors. The antioxidant activity test demonstrated 50% inhibition at a concentration of around 30 mg/mL, with two samples deviating due to the presence of impurities. The anti-inflammatory activity test showed inhibition ranging from 30 to 45%. Čebele strup kozmetika lastnosti kemija proteini antioksidativnost protivnetnost krema hidrogel Bees venom cosmetics properties chemistry proteins antioxidant anti-inflammatory cream hydrogel true true false [K. Glušič] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-08-28 16:27:00 2025-09-29 16:02:27 2025-10-31 03:22:38 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IX, 33 str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 54.04:638.178(043.2) 255363587 VS_Glusic_Klara_2025.pdf VS_Glusic_Klara_2025.pdf 1 AF16029A5CE6D51D006FB6C84153E0D1 902bd9f18332bdf4c5c143f9330c3d360e48cadf2803ec454ff391947b396730 e3c67dfb-9a9f-11f0-9a69-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=199241 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0