20.500.12556/DKUM-95487 Analiza genetske raznolikosti akcesij malin (Rubus idaeus L.) iz genske banke Fakultete za kmetijstvo in biosistemske vede z uporabo mikrosatelitskih markerjev (SSR) magistrsko delo Analysis of genetic diversity in raspberry (Rubus idaeus l.) accessions from the plant gene bank of the Faculty of Agriculture and Life Sciences using microsatellite (SSR) markers Maline (Rubus idaeus L.) so priljubljena sadna vrsta, primerna za svežo uporabo in predelavo. Pridelava je v zadnjem desetletju močno narasla, kar je spodbudilo potrebo po razvoju sort, odpornih na bolezni in škodljivce ter prilagojenih specifičnim rastnim pogojem. Večina današnjih kultivarjev izvira iz majhnega števila starševskih rastlin, zato je ključno, da se za potrebe žlahtnjenja zagotovi gensko raznolik material. Pomembno vlogo pri tem imajo genske banke, katerih uspešnost temelji na ustrezni karakterizaciji in natančni identifikaciji akcesij ter preprečevanju podvajanj. Namen magistrskega dela je bil z uporabo mikrosatelitskih markerjev preveriti genetsko sorodnost med akcesijami malin, vključenimi v gensko banko Fakultete za kmetijstvo in biosistemske vede, ter oceniti uporabnost izbranih mikrosatelitskih lokusov za tovrstne analize. Na osnovi podatkov kapilarne elektroforeze smo z metodo »neighbor-joining« (NJ) izdelali dendrogram, ki je omogočil vizualizacijo genetskih odnosov med akcesijami. Rezultati so razkrili prisotnost večjega števila duplikatov, večinoma v skupinah po 2–3 identične akcesije, v enem primeru pa jih je bilo zaznanih celo 8. Kljub visoki informativnosti uporabljenih markerjev so naši rezultati omejeni zaradi majhnega števila analiziranih mikrosatelitskih lokusov (4), kar zmanjšuje natančnost zaključkov. Raspberries (Rubus idaeus L.) are a popular fruit, as they can be used fresh or processed in a variety of products. The production of raspberries has seen a significant incline in the past decade. Therefore, there is an evergrowing need for hardy varieties, adapted to specific growing locations. Because of the origin of most modern varieties being from a small number of parent plants, it is important for genetically diverse breeding material to be made accesible. Gene banks play an important role in the preservation of genetic diversity. Accessions in gene banks must be correctly characterised, identified, marked, and be free of duplicates. The purpose of this master's thesis was the analysis of genetic relatedness among raspberry accessions stored at the Faculty of Agriculture and Life Sciences gene bank via the use of microsatellite markers. One of the goals was also to analyse the utility of the chosen microsatellite markers for future research. Data produced from a capillary electrophoresis was used to construct a dendrogram, using the Neighbor-Joining (NJ) method. The dendrogram made it possible to visualize genetic relationships between accessions. The results showed a great number of discovered duplicates, mainly in groups of 2 to 3 accessions, 8 in a single case. However, methodological limitations due to a small number of analysed microsatellite loci (4) prevented definitive conclusions. genska banka genetska sorodnost malina mikrosatelitski markerji žlahtnjenje malin gene bank genetic relatedness raspberry microsatellite markers raspberry breeding true true false M. Petek Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-09-22 11:01:39 2025-10-29 13:06:34 2025-10-30 03:15:36 0000-00-00 00:00:00 2025 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IX, 81, [1] str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.71:631.524(043)=163.6 255168003 MAG_Petek_Miha_2025.pdf MAG_Petek_Miha_2025.pdf 1 90923F486F2CD44573D821CFC3677CA8 411a3e50cfda977ca90490eb253cac0f01a697638f1a5c981ed347018b8f5854 ebe4c53d-9798-11f0-88a2-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=198953 Fakulteta za kmetijstvo in biosistemske vede 0 0 0