20.500.12556/DKUM-95867 Chemo-enzymatic synthesis of viscoelastic pseudopeptidoglycan networks Bacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and 1H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response. biomaterials bacterial peptidoglycans (PGs) chemo-enzymatic synthesis biomateriali bakterijski peptidoglikani (PG) kemo-encimska sinteza true false true ACS Publications Angleški jezik Slovenski jezik https://pubs.acs.org/doi/10.1021/acs.bioconjchem.5c00014 Članek v reviji 2025-11-03 12:39:38 2025-11-03 12:39:38 2025-11-04 03:34:50 0000-00-00 00:00:00 2025 0 0 str. 1933-1942 no. 9 Vol. 36 June 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-06-24 576:60 254908163 10.1021/acs.bioconjchem.5c00014 1520-4812 chemo-enzymatic-synthesis-of-viscoelastic-pseudopeptidoglycan-networks.pdf chemo-enzymatic-synthesis-of-viscoelastic-pseudopeptidoglycan-networks.pdf 1 439AEC2216CF31FD3F1B8F94B1394C94 057ec06c177a0c418beced7ba548544b50103e525e45ba40d6fcbd29f417c750 4e00d905-b8aa-11f0-b13b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=200350 https://pubs.acs.org/doi/10.1021/acs.bioconjchem.5c00014 0 555a830b-b8aa-11f0-b13b-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=200351 Fakulteta za strojništvo 0 0 0