20.500.12556/DKUM-96175 Macromolecular inks of nanocellulose-alginate for direct-ink-writing functional scaffolds enriched with curcumin extracts We present a scalable platform for the fabrication of multifunctional bioactive materials by integrating, polysaccharide-based matrices, green extraction and additive manufacturing. In this work, bioactive 3D-printed structures were fabricated using inks composed of nanofibrillated cellulose (NFC), alginate (Alg), and curcumin extracts. Bioactive compounds were efficiently recovered from turmeric via solvent-free supercritical carbon dioxide extraction, yielding up to 6.57 mg/100 g curcuminoids and 232.45 mg/100 g total phenols. The extracts exhibited robust antioxidant activity (DPPH• IC50: 23.24 mg/mL; ABTS+• IC50: 3.59 mg/mL) and broad-spectrum antimicrobial efficacy against Staphylococcus aureus, Escherichia coli, Candida albicans, and Pseudomonas aeruginosa (MIC: 9.38 mg/mL). Incorporation into NFC-Alg inks exhibited excellent shear-thinning properties suitable for direct-ink-writing 3D printing. Post-printing CaCl2 crosslinking further reinforced the hydrogel network, enhancing mechanical robustness and shape fidelity. The resulting constructs featured a highly porous, grid-like architecture with intricate surface morphology, highlighting significant potential for biomedical and tissue engineering applications. nanocellulose alginate curcumin extract supercritical-CO2 antioxidant antimicrobial properties 3D-printing nanoceluloza alginati ekstrakt kurkumina superkritični ogljikov dioksid antioksidanti antimikrobne značilnosti 3D tiskanje true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/international-journal-of-biological-macromolecules © 2025 The Authors Članek v reviji 2025-12-08 11:32:51 2025-12-08 11:32:51 2025-12-09 03:10:38 0000-00-00 00:00:00 2026 0 0 13 str. Part 1, ǂ[article no.] ǂ149261 Vol. 335 Jan. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-08-07 2025-11-24 2025-11-25 66 259537923 10.1016/j.ijbiomac.2025.149261 1879-0003 1-s2.0-S0141813025098186-main.pdf 1-s2.0-S0141813025098186-main.pdf 1 591C61E4AEAFB56ABCCD5A3FAF6EEA83 273077f59e0587c1c95bb1d9a434f5264cd7ae34d43895daa353083a49a2f22b 976a0c32-d421-11f0-acea-00155d000105 https://dk.um.si/Dokument.php?lang=slv&id=201204 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0