20.500.12556/DKUM-96319 Bioreactor parameters and systems for cultured meat production Cultured meat (CM) is an emerging technology involving the use of animal cells to produce meat in vitro. It has the potential to replace or complement traditional meat production, offering a sustainable and ethical alternative to farmed animals. CM could provide sufficient meat to satisfy the growing demand for animal-based protein while addressing the negative impact of traditional livestock agriculture such as disease transmission, antibiotic misuse, poor animal welfare, and greenhouse gas emissions. But efficient industrial bioreactor systems, low-cost media, and high producing suitable cells are needed for CM technology to compete with or supplement conventional meat production. Bioreactors provide a controlled in vitro environment for the supply of nutrients and stimulus to the cells, which is necessary for cell expansion and differentiation on a large scale. In this review, we discuss the state of the art in bioreactor systems used for CM production, highlighting their characteristics and applications, advantages and disadvantages, the effect of key process parameters, and scale-up strategies. We also consider key performance indicators for the bioreactor-based large-scale production of CM. bioreactor design cell differentiation cell expansion cultured meat scale-up stem cells true true true Elsevier Angleški jezik Angleški jezik Znanstveno delo 2025-12-18 08:28:17 2025-12-18 08:37:53 2025-12-19 03:18:33 0000-00-00 00:00:00 2025 0 0 Str. 1-14 št. članka 100796 Letn. 12 Dec. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-07-28 2025-02-07 2025-12-01 60 257977091 10.1016/j.fufo.2025.100796 2666-8335 RAZ_Ngwa_Che_Julius_2025.pdf RAZ_Ngwa_Che_Julius_2025.pdf 1 D8AFFA55923BC7C09B99B073745FA0D0 dc65400dc24e3b2a6206f610af85aa1923be584cc77df6d687a4c8418f721fe8 014c02e7-dbe4-11f0-af12-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201525 https://doi.org/10.1016/j.fufo.2025.100796 0 4b5c3179-dbe4-11f0-af12-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201526 https://www.sciencedirect.com/science/article/pii/S2666833525002552 1 1ff814dc-dbe3-11f0-af12-00155d580e02 https://dk.um.si/Dokument.php?lang=slv&id=201524 Medicinska fakulteta 0 0 0